Research On The Expression Of PPARs In Peripheral Blood Mononuclear Cells From Patients With PCOS

Objective: To study the expression of peroxisome proliferation-activated receptors,PPARs(PPARα,PPARβ and PPARγ)in peripheral blood mononuclear cells from patients with polycystic ovary syndrome(PCOS).To analyze the relationship between the expression of PPARs in peripheral blood mononuclear cells and the clinical characteristics of PCOS disease.Methods:Polycystic ovary syndrome was diagnosed according to the Chines diagnose PCOS 2011 standard.The PCOS group consists of 21 patients with polycystic ovary syndrome(PCOS)collected in Zhongshan Hospital of Dalian University gynecology outpatient department from January 2017 to APril 2018.The control group consists of 30 women with normal menstruation,without clinical manifestations of the androgen excess,hyperandrogenism,normal gynecological color ultrasound examination,No metabolic diseases in our hospital.Collect general information about the research objects: height,weight,acne,hirsutism,etc.All the research objects were collected the fasting venous blood on the early follicular phase or the amenorrhea phase.The research objects were compared with the values of androgen,luteinizing hormone,follicle stimulating hormone,estrogen,blood glucose and insulin levels.Peripheral blood mononuclear cells were extracted from by gradient density centrifugation.The expression of PPARs m RNA in peripheral blood mononuclear cells was detected by SYBRGreen II real-time quantitative polymerase chain reaction.Ct value was used to compare the expression level of PPARs(PPARα,PPARβ,PPARγ)m RNA in peripheral blood mononuclear cells between two groups.Analysis was used to analyze the correlation between the expression of PPARs in peripheral blood mononuclear cell and fasting blood glucose,fasting insulin,androgen,luteinizing hormone in PCOS group.Results:1.The age of PCOS group was 21~35 years old,The average age was 27.47±3.62.15 cases of rare menses(71.4%).Two cases of amenorrhea(9.5%).4 cases with irregular uterine bleeding(19%).Hairy 7 cases(33%).12 cases of acne(57%).Color ultrasonography prompt: 4 cases of polycystic ovary(19%).The age of control group was 23 ~ 35 years,average age: 28.53±3.06.Menstrual cycles are normal.Comparison PCOS group to the control group,the age and the body mass index were no statistically significant difference(all P>0.05).2.The androgens and luteinizing hormone were all higher in PCOS group than in the control group(all P<0.05).However,there was no statistically significant difference in the estrogen,follicle stimulating hormone between the PCOS group and the control group(all P>0.05).3.The fasting blood glucose and fasting insulin were all higher in PCOS group than in the control group(all P<0.05).4.The expression level of PPARα,PPARβ and PPARγ m RNA in peripheral blood mononuclear cells in PCOS group were all lower than in the control one(all P<0.05).5.According to the results of linear correlation analysis:In the PCOS group INS(r=-0.662),T(r=-0.467)were significantly negatively correlated with the expression of PPARγ m RNA in peripheral blood mononuclear cells(P<0.05).In the PCOS group FPG,INS,T and LH had no significant correlation with the expression of PPARα,PPARβ m RNA(P>0.05).Conclusions: 1.The abnormal expression of PPARα,PPARβ and PPARγm RNA in peripheral blood mononuclear cells may be related to the clinical characteristics of PCOS disease.2.PPARγ m RNA expression in peripheral blood mononuclear cells of PCOS patients was negatively correlated with androgen and fasting insulin.The down-regulated expression of peripheral blood mononuclear cells(PPAR)may promote the occurrence of hyperinsulinemia and hyperandrogenemia in patients with polycystic ovary syndrome.