Therapeutic Effects And Treatment Mechanism Of Cheng’s Juanbi Decoction On Adjuvant Arthritisrats

Aim:To observe the effects of Cheng’s Juanbi Decoction on the pain threshold and ear edema of mouse,and use carrageenin-induced inflammatory rat model and adjuvant arthritis rat model,to explore its anti-inflammatory and analgesic effects and its mechanism of action in treating and relieving arthritis.Methods:(1)for the pain threshold test and ear edema test,mice were randomly divided into five groups:model group,aspirin group(0.05g/kg),CSJBT-L group(5.10 g/kg),CSJBT-M group(10.20g/kg)and CSJBT-H group(20.40 g/kg).Mice were treated with CSJBT by intragastric administration once a day for 7 days,pain threshold and ear edema was measured to evaluate the analgesic effect of CSJBT on mice.(2)for the acute inflammatory rats test,rats were randomly divided into six groups:normal group,model group,glycosides of tripterygium wifordii group(0.01 g/kg),CSJBT-L group(2.55 g/kg),CSJBT-M group(5.10g/kg)and CSJBT-H group(10.20 g/kg).Rats were treated with CSJBT by intragastric administration once a day for 14 days.One hour after the last intragastric administration,except the normal group,rats were injected with 0.1 m L carrageenan into the left hindpaw.Hindpaw swelling was measured at 1h,2 h,4 h and 6 h after inflammation.(3)for the chronic inflammatory rats test,grouping of rats was the same to that of acute inflammatory rats test.rats were induced by intradermal injection of 0.1 mL CFA in the left hindpaw,except the normal group.The intragastric administration was begin on the 15 d after inflammation,rats were treated with CSJBT once a day for 14 days,one hour after the last intragastric administration,the primary and secondary side-foot swell and flexor-joint pain scores were measured.After taken blood from rat abdominal aorta,whole blood viscosity,plasma viscosity,erythrocyte sedimentation rate,hematocrit and whole blood high reduction viscosity were measured.Flow cytometer was used to detect the effect of CSJBT on Th17 cells(CD4~+IL-17A~+)and Treg cells(CD4~+CD25~+Foxp3~+)and the ratio between them.Results:(1)CSJBT high dose(20.4 g/kg),medium dose(10.2 g/kg)group significantly increased the pain threshold and decreased ear edema in mice with inflammation(P<0.05).(2)CSJBT high dose(10.20 g/kg)and medium dose(5.10 g/kg)significantly reduced the foot swell rate of rats with acute inflammation(P<0.05).(3)CSJBT high dose(10.20 g/kg)and medium dose(5.10 g/kg)significantly decreased the foot swell rate and flexor-joint pain scores of rats with chronic inflammation(P<0.05),CSJBT medium dose(5.10 g/kg)reduced whole blood viscosity.CSJBT high dose(10.20 g/kg)and medium dose(5.10 g/kg)significantly increased the proportion of Treg cells in blood and spleen lymphocytes of rats(P<0.05),and decreased the proportion of Th17 cells and the ratio of Th17/Treg(P<0.05).Conclusion:CSJBT has good therapeutic effects on AA rats,and can maintain the balance of Th17/Treg which suggests the treatment mechanisms are related to regulating the immune function.