The Effect Of Ginkgolide B On Modulating Inflammatory And Oxidative Response Of Activated Glia Cells Caused By Lipopolisccharide

Objective: To select the best inflammatory model from SD rats mixed glial cells,C57 mice mixed glial cells and kunming mice mixed glial cells.To explore the effect of ginkgolide B on the anti-inflammation and antioxidation of glial cells caused by LPS.Methods: We use LPS as inducers,and NO、IL – 1β、Cox-2 、i NOS as antiinflammatory antioxidant index,and use Greiss assay to detect the expression of NO.Western blot could detect the expression of protein of IL – 1β,Cox-2,and i NOS protein,finally we could compare mixed glial cells from SD rats,C57 mice mixed glial cells,kunming mouse mixed glial cells and select the best inflammation model from three mixed glial cells;To explore the effect of ginkgolide B on the antiinflammation and antioxidation of LPS mediated glial cells.Results: We cut the three kinds of mice(C57 mice SD rats and kunming mouse)and get the brain,then cultivating mixed glial cells.After immunofluorescence test astrocytes,the results show that most of the cells are glial cells,so we can use it for the experiment.We use LPS to mediate three mixed glial cells of rats after 24 h,then Greiss assay detect the concentration of NO of the supernatant.compared with the control group,the quantity of NO of LPS group increased significantly.And the LPS group of SD rats released the highest concentration of NO.After extracting the protein,Western blot was used to detect the expression of IL-1β,Cox-2 and i NOS in three mice: compared with the blank control group,the expression of Cox-2 protein,i NOS and IL-1β in the LPS group of the three mice increased significantly.The results showed that LPS could successfully stimulate the release of inflammatory cytokines in three kinds of mice,which could be used in the study of AD inflammation model,and the SD rats were more sensitive.Primitive mixed glial cells with SD rats as the AD inflammation model to explore the anti-inflammatory of aspirin,ginkgo lactone B antioxidant effect,the results showed that aspirin and LPS altogether 12 h after training,show no evidence to LPS stimulation mixed glial cells release NO inhibition;After a total of 24 h and 36 h,LPS induced its cells to produce NO.After 12 h of ginkgolide B and LPS,NO inhibitory effect on the release of NO by LPS was shown,and the release of NO was significantly reduced after a total incubation of 24 h and 36 h.Ginkgolide B synergistic SB(p38MAPK inhibitor)and LPS co-incubate 12 h,24 h and 36 h can effectively inhibit the release of LPS.Conclusion: compared with kunming mice and C57 mice,the mixed glial cells of SD rats were more sensitive to LPS-induced inflammatory response and were more suitable for the inflammatory response model.Ginkgolide B(10μM)and LPS(100ng/ml)were co-incubated with cells,which could reduce the concentration of NO release in LPS induced SD rats.Aspirin(1μM)and LPS(100 ng/ml)were coincubation with cells for 24 h and 36 h could reduce the concentration of NO release of LPS-induced SD rats.The co-incubation of Ginkgolide B with SB、LPS for 12 h、24 h、and 36 h was effective in reducing the concentration of NO release of the LPSinduced SD rat mixed glial cells.