| ObjectiveChronic hepatitis B virus(HBV)infection is one of the most common public health problems worldwide.Asia Pacific region accounts for 3/4 of chronic HBV-positive subjects worldwide,China is one of the countries which with the largest population of HBV infection.Long-term chronic HBV infection increases the risk of liver cirrhosis and hepatocellular carcinoma(HCC),but the mechanism is not fully determined.Recent studies have reported that HBV related susceptibility and progression are affected by virus,genetic polymorphism and environmental factors.Transmembrane 6 superfamily member 2(TM6SF2)single nucleotide polymorphisms(SNPs)has been identified as a determinant of hepatic fat content that triggering steatohepatitis and fibrogenesis.It may be the key factor in the clinical outcome of liver disease.Many researchers have shown a correlation between this gene polymorphismsthe and non-alcoholic fatty liver disease(NAFLD),alcoholic liver disease(ALD),chronic hepatitis C(CHC)and other diseases.However,few reports have been reported on the link between the TM6SF2 gene rs58542926 polymorphism and HBV infection related diseases.Therefore,we studied the distribution of rs58542926 polymorphism of TM6SF2 gene in Chinese Han population infected with HBV,and explored the correlation between TM6SF2 gene polymorphism and HBV related cirrhosis in China Han population.MethodsThis association study was designed as a retrospective study,including 399 patients with chronic HBV infection and 179 healthy controls.All subjects were ethnic Han Chinese.HBV-infected patients recruited from the Qingdao municipal hospital were further classified into HBV carriers patients(n=160)and HBV-related LC patients(n=239)according to the guidelines for the diagnosis of chronic HBV carriers and LC.Healthy controls recruited from physical examination center during the same period.All subjects were collected for medical history,biochemical data and blood samples.Human genomic DNA was extracted from whole blood using purification kit according to the manufacturer’s instructions,application of polymerase chain reaction(PCR)as TM6SF2 rs58542926 genotypes,that definite the distribution of genotype and allele.Comparing the general information of the case group and the control group,rs58542926TM6SF2 genotype and allele frequency distribution difference comparison.Using logistic regression to determine the relationship between genetic mutations associated with HBV and LC.Data analysis was applied to statistical software SPSS 22.0.ResultsThe genotypes of CC,CT and TT in the TM6SF2 gene rs58542926 were detected in each group: control group CC: 89.9%,TC: 10.0%,TT: 0%;HBV carriers group CC:88.7%,TC:11.2%,TT: 0%;LC group CC: 87.4%,TC:12.1%,TT: 0.4%.According to the Hardy-Weinberg(HWE)genetic balance test,the distribution of gene polymorphisms in each group was in line with the(H-W)balance rule.There were no significant differences between the genotype and allele frequencies of the TM6SF2rs58542926 gene polymorphisms,and risk of CHB and HBV-related LC(P>0.05).Logistic regression showed that,comparing with the control group,the risk of HBV-carriers was(OR=1.13,95 % CI: 0.56~2.26,P>0.05),and the risk of LC was(OR=1.28,95%CI: 0.69~2.38,P>0.05)in T gene carriers.After adjusted for age and sex,the risk of HBV-carriers was(OR=0.96,95%CI: 0.46~2.00,P>0.05),and the risk of LC was(OR=0.84,95%CI: 0.44~1.59,P>0.05)in T gene carriers.Comparing with HBV-carriers,the risk of LC was(OR=1.13,95 % CI: 0.60~2.10,P>0.05)in T gene carriers,adjusted for age and sex,the risk of LC was(OR=0.87,95%CI: 0.46~1.64,P>0.05).It was significantly associated with the level of serum LDL in variant genotype than the wild genotype in LC(P <0.05).Conclusions The polymorphisms of TM6SF2 gene rs58542926 is not associated with risk of HBV related liver cirrhosis in Chinese Han population. |
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