Quantitative Assessment Of The Effects Of Reducing Agents On Biological Macromolecules And The Possibility Of Oxidative Damage Repair

Objective:We quantitatively assessed the influence of reducing agents on biological macromolecules and explored the possibility of repair of oxidative damage by using reducing agents.Methods:1.Enzyme linked immunosorbent assay（ELISA）was used to quantitatively analyze the effect of reduction agenton hepatitis B surface antibody activity.2.Native-PAGE and SDS-PAGE were used to analyze the effects of reducing agents on the spatial structure and molecular structure of serum proteins.3.The effects of reducing agents on enzymes and DNA were analysised.4.The agglutination test was used to compare the agglutination titer and agglutination intensity to quantitatively analyze the effect of vitamin C on the antigenicity of Salmonella typhi;The effect of vitamin C on the activity of hepatitis B surface antibody was quantitatively analyzed by enzyme linked immunosorbent assay.5.Oxidizing agents are first treated with Bacillus typhus to cause oxidative damage,and then treated with reducing agent,by comparing the agglutination strength of the experimental group and the control group,the possibility of the repair of the oxidative damage was discussed;Oxidizing agents are first treated with hepatitis B surface antibody to cause oxidative damage,and then treated with reducing agent,comparing the OD value of experimental group and control group to explore the possibility of oxidative damage to be repaired by enzyme-linked immune osorbent assay;The serum of 90 elderly patients with hepatitis B surface antibody was treated with reducing agent.The serum of the control group was treated with sodium chloride and the enzyme linked immunosorbent assay was used to measure the antibody activity,and the possibility of reducing the oxidation damage caused by aging was discussed.Result:1.Effect of reducing agent on antibody activity:After the antibody was treated with potassium iodide and sodium thiosulfate the inhibition rate of in the experimental group was not significantly different from that in the control group.After the antibody was treated with vitamin C and ammonium ferrous sulfate,the inhibition rate of the experimental group was significantly different from that of the control group.2.Effect of reducing agent on the structure of serum protein:The protein bands in the（NH₄）₂Fe（SO₄）₂ group were deficient and fuzzy compared with the NaCl group.The protein bands for the Vc,KI,and Na₂SO₃ groups showed no differences compared with the NaCl group.3.The effects of reductants on enzyme and DNA:The inhibitory rates of vitamin C,potassium iodide and sodium thiosulfate on the enzyme and DNA in the experimental group were not significantly different from those in the control group.The inhibition rate of ammonium ferrous sulfate on enzyme and DNA in the experimental group was significantly different from that in the control group.4.Immunological toxicology of vitamin C:Salmonella typhimurium was treated with vitamin C at the concentration of 30mmol/L,the inhibition rate was 96.6%,the inhibition rate was 81.4%at the concentration of 15mmol/L,the inhibition rate was 37.3%at the concentration of 7.5mmol/L;The ID50 of vitamin C for hepatitis B surface antibody is 6.63mmol/L.5.Salmonella typhi was treated with hydrogen peroxide,and then treated with reducing agent.The agglutination strength of Salmonella typhi and antiserum was improved.The hepatitis B surface antibody was treated with potassium permanganate,then treated with reducing agent,the activity of hepatitis B surface antibody was increased;There was no significant difference in the activity of hepatitis B surface antibody between the serum of 90 year old people treated with reducing agent or sodium chloride.Conclusion:1.Ammonium ferrous sulfate has a inhibitory effect on the activity of antibodies,enzymes and DNA,and has a destructive effect on the serum protein structure in the range of the experimental concentration;Vitamin C has an inhibitory effect on the activity of antibody,and there is no effect on the activity of enzyme and DNA and the structure of serum protein in the range of the experimental concentration;Potassium iodide and sodium thiosulfate had no effect on the activity of antibody,enzyme and DNA and the structure of serum protein in the range of the experimental concentration.2.Vitamin C has an inhibitory effect on the antigenicity of Salmonella typhi and the activity of hepatitis B surface antibody.3.Potassium iodide and sodium thiosulfate have a certain repair effect on oxidative damage caused by the oxidant on Bacillus typhus;Potassium iodide and sodium thiosulfate have a certain repair effect on oxidation damage caused by the oxidizing agent on hepatitis B surface antibody;Potassium iodide and sodium thiosulfate have no repair effect on oxidative damage caused by long-term oxidative stress caused by aging.