Clinical Significance Of Tissue Factor–bearing Microparticles Procoagulant Activity And Antithrombin Ⅲ In Thalassemia Patient

objectiveWe aim to explore whether there is a risk factor that may lead to ahypercoagulative state in thalassemia patients by measuring Tissue factor–bearing microparticles（TF⁺MP）procoagulant activity、coagulation and anticoagulation function、fibrinolytic function、endothelial function and platelet（PLT）count of thalassemia patients.Methods71 thalassemia patients and 20 health was involved.Tissue factor-microparticles procoagulant activity was measurd by chromogenic substrate method,the levels of TF、TFPI、PC、PS、AT-Ⅲ、t-PA、TAFI、sE-sel、sICAM-1、sTM was measured by ELISA assay.Result Comparing with the Control group（C group）:the level of AT-Ⅲ in thalassemia group（T）andβ-thalassemia major group（TM）were lower（P<0.05）;In TM,AT-Ⅲ level was positive correlated with PLT(r_PLT=0.37,P<0.05).;the levels of TF and sICAM-1 inα-thalassemia intermedia group（TA）were lower（P<0.01）;TF⁺MP procoagulant activity in splenectomy group（S）andβ-thalassemia intermedia group（TI）were higher（P<0.05）and independent positive correlated with s ICAM-1 and AT-Ⅲ respectively(r _sICAM-1=0.84,P<0.05;rp_AT-Ⅲ=0.77,P<0.05);The level of PLT in S group was higher than C group and NS group(P_C<0.05P _NS<0.01);TF and sICAM-1 of the normal liver function group were lower than C group(P _TF<0.01,P _sICAM-1<0.05).TF⁺MP activity between the normal and abnormal liver function group were significian different.There are not Significant correlations of any other indexes in corresponding group.Conclusionβ-thalassemia major patients presence hepatic damage and reduction of anticoagulant substance;TF⁺MP procoagulant activity abnormally elevated in splenectomy thalassemia patients as also asβ-thalassemia intermedia petients;platelet level abnormally elevated in splenectomy thalassemia patients.All these factors above may be the risk factors for the formation of hypercoagulative state and thrombosis in thalassemia patients.But the reduction levels of TF and sICAM-1 inα-thalassemia intermedia petients and petients of normal liver function may be the protective Factors of thrombosis.