A Study Of Correlation Between IL-6,TGF-β,IL-33,S100A8 And Radiation Induced Lung Injury

Objective: To analyze the relationships between four cytokines(IL-6,TGF-β,IL-33,S100A8)of the patients with non-surgical esophageal squamous cell carcinoma and radiation induced lung injury(RILI).Methods: We retrospectively analyzed 60 patients diagnosed as non-surgical esophageal squamous cell carcinoma who were treated with radical three-dimensional conformal or intensity-modulated radiotherapy in our hospital between 2015 and 2017,with a median follow-up of 7 months for RILI.50-50.4 Gy of radiotherapy was completed within 5-6 weeks.The sample of patients’ peripheral blood was collected in each time points as protocol,and the level of cytokines were tested by Elisa.Results: Among all examined baseline features in RILI and Non-RILI groups,only pulmonary function was significantly associated with the presence of RILI(P<0.05).There was no significant difference between gender,age,TNM stage,radiation dose,KPS score,smoking history and the presence of RILI(P>0.05).The total absolute concentration of cytokines after radiation were not remarkably changed comparing with the level before radiation(P > 0.05).Comparing to pre-radiation,the absolute concentration of IL-6 was significantly increased in 12 and 24 weeks after radiation began(P<0.05),but the relative concentration was only significantly increased in 12 weeks after radiation began(P<0.05).TGF-β was not significant changed before and after radiation in RILI group(P>0.05).The absolute and relative concentration of IL-33 were notably changed in 12 and 24 weeks after radiation began in RILI group(P<0.05).The absolute concentration of S100A8 was significantly increased in 12,24 and 28 weeks after radiation began(P < 0.05),and the relative concentration was significantly increased in 12 and 28 weeks after radiation began(P<0.05).In ROC curve analyze,IL-16,IL-33 and S100A8 of 12 and 24 weeks after radiation began were well performing in sensibility and specificity in predicting the occurrence and development of RILI(P<0.05).Multivariate Logistic showed that the concentration of IL-16 and IL-33 were independent markers of occurrence and development of RILI(P<0.05).Conclusion: The concentration of IL-6,IL-33 and S100A8 in patients’ serum with non-surgical esophageal squamous cell carcinoma are significantly increased after radiation comparing to pre-radiation.IL-6 and IL-33 are associated with the occurrence and development of RILI.