Effects Of Ivabradine Hydrochlorid On Myocardial Fibrosis In Rats With Chronic Heart Failure

ObjectiveTo investigate the effects of Ivabradine hydrochlorid(Iva)on myocardial fibrosis(MF)in rats with chronic heart failure(CHF).MethodThe Forty-four wistar rats were randomly divided into sham-operation group,control group(NC)group,Iva group and positive control druggroup.Except for the control group,the other 3 groups of rats were used to constract the heart failure model by narrowing the abdominal aorta.After the six weekend,the left ventricular end-diastolic pressure(LVEDP)of each rat was measured,LVEDP≥15 mmHg as modeling-standards.Iva group was fed 10mg/kg Iva per day and 10 mg/kg betaloc for betaloc group each day.The sham operation group and the blank control group had 10 mg/kg of normal saline.Left ventricular end-diastolic pressure and the expression of connective tissue growth factor(CTGF)as well as superoxide dismutase(SOD)content in myocardium were also determined.HE and Masson staining can also dectect the degree of myocardial fibrosis too.ResultsCompared with the sham-group,the LVEDP of the NC group was significantly increased(P<0.01),the content of SOD was significantly decreased(P<0.01)and the expression of CTGF was significantly increased(P<0.01),the degree of myocardial fibrosis is extremely serious.After,treatment with Iva,LVEDP and the content of SOD was significantly increased(P<0.01),however,the expression of CTGF was significantly inhibited(P<0.01).The degree of myocardial fibrosis was obviously alleviated.ConclusionIva can significantly reduce the CHF rat heart LVEDP,significantly increase the SOD content in myocardial tissue,inhibit myocardial tissue CTGF expression,which have inhibitory effects on rats with chronic heart failure.