Expression And Significance Of IL-31、IL-33 And CCL27 In The Serum Of Patients With Psoriasis Vulgaris

BackgroundPsoriasis is a chronic inflammatory dermatosis characterized by epidermal hyperproliferation and inflammation.Psoriasis is a systemic disease that can cause a decline in quality of life,especially if accompanied by pruritus can cause discomfort.Psoriasis can be divided into vulgar,pustular,erythrodermic and psoriatic arthritis,of which about 90% is psoriasis vulgaris.But the potential mechanism underlying its pathogenesis remains to be fully deciphered.Interleukin-31 is a recently discovered four-helix bundle cytokine that is important for both innate and adaptive immunity in skin.IL-31 signal transduction is through a heterodimeric receptor consisting of IL-31 receptor alpha(IL-31RA)and oncostatin M receptor beta(OSMR).Studies have shown that overexpressed IL-31 in transgenic mice induce pruritus,but the correlation between IL-31 and the pruritus in patient with psoriasis vulgaris is stillpoorly studied.IL-33 is one such damage-associated molecular patterns(DAMP),which was recently identified as a member of the IL-1 family,and binds to a heterodimeric receptor comprising ST2 and IL-1 receptor accessory protein.IL-33 acts on ST2-expressing cells such as Th2 cells,mast cells,DCs and eosinophils.IL-33 expression is increased in lesional skin in patients with psoriasis,but serum levels in patients with psoriasis have not yet been fully studied.CCL27,also known as ESkine,LP,ILC or CTACK,is a member of the CC chemokine subfamily and it is a ligand to CCR10.The binding of CCL27 to CCR10 on T cells promotes their migration to the skin,where they can then participate in local immune responses.The expression of CCL27 in lesional skin of psoriasis vulgaris is significantly higher than that of normal skin,however the serum levels of CCL27 in patients of psoriasis remains to be fully studied.ObjectiveThe aim of this investigation was to quantify the expression levels IL-31,IL-33 and CCL27 in the serum of patients with psoriasis vulgaris and examine the correlation between the serum levels and disease severty(PASI).Then determine the roles of these cytokines in pathogenesis of psoriasis vulgaris.Methods73 patients with psoriasis vulgar(31cases in static,42 cases in progressive)together with 73 healthy control subjects were selected and enrolled in the study.Measureing the expression levels of IL-31,IL-33,CCL27 in serum by enzyme-linked immunosobent assay(ELISA)and analysised the relationship of the serum levels.Results1.The expression levels of IL-31,IL-33 and CCL27 in the serum of patients with psoriasis vulgaris were higher than those in healthy control,the difference was statistically significant(P<0.05).The expression levels of IL-31 and CCL27 in patientsserum in progressive stage were higher than in static stage,the difference was statistically significant(P<0.05),but the expression levels of IL-33 in patients serum in progressive stage was statistically no difference with that in static stage(P>0.05).2.The expression levels of IL-31,IL-33 and CCL27 in the serum of patients with psoriasis vulgaris were positively correlated with their PASI score(P<0.05).ConclusionsThe expression levels of IL-31,IL-33 and CCL27 in the serum of patients with psoriasis vulgaris were significantly higher than those in healthy control subjects,and it was positively correlated with their PASI scores,which suggested that IL-31,IL-33,and CCL27 were involved in the pathogenesis of psoriasis vulgaris as proinflammatory factors.