The Correlation Between The Expression Of Negative Regulatory Cells CD4~+ CD25~+ T Cells And CD5~+ CD19~+ B Cells In Peripheral Blood Of Systemic Lupus Erythematosus Patients With Combined Bacterial Infection

Objective:To understand the expression of the two negative regulatory cells CD4~+CD25~+Tcells and CD5~+CD19~+B cells in the peripheral blood of patients with systemic lupus erythematosus(SLE);To investigate the relationship between two negative regulatory cells with bacterial infection in patients with SLE,and the relationship between the two negative regulatory cells with the disease activity in patients with SLE.And then to provide clinical prediction of lupus patients with bacterial infection and lupus disease activity indicators.Method:We collected 50 patients with Lupus,among them,18 were infected with bacterial infection and 32 were non-combined bacterial infection;Normal control group of 17 people;In the non-co-infection of lupus in 32 people were divided into stable group of 12 people,active group of 20patients with lupus.Extraction of peripheral anticoagulant blood,Use flow cytometry to detect the percentage of CD4~+CD25~+Tcells and CD5~+CD19~+B cells in lymphocytes in peripheral blood,at the same time collecting lupus patients laboratory tests,test results and clinical symptoms,to get the SLEDAI score.Result:1.The percentage of CD4~+CD25~+Tcells in peripheral blood of patients with lupus accounted for lymphocytes is(12.44±6.46)%compared with the normal control group(11.47±2.57)%,P=0.383,>0.05,the difference was not statistically significant.2.The percentage of CD4~+CD25~+T cells in peripheral blood in patients with lupus with bacterial infection(10.46±6.37)%was lower than that in patients with non-combined bacterial infection of lupus(14.37±6.02)%,P=0.018,<0.05,the difference was statistically significant.3.The SLEDAI score in patients with lupus combined bacterial infection(12.94±5.84)was higher than that in non-patients with lupus(8.08±3.98),P=0.001,<0.05,the difference was statistically significant.4.The percentages of CD5~+CD19~+Bcells in peripheral blood of patients with lupus combined bacterial infection(2.41±2.95)%compare with that of patients without bacterial infection of lupus(1.79±1.9)%,P=0.316,>0.05,The difference was not statistically significant.5.The level of CD4~+CD25~+T cells in lymphocytes in peripheral blood of patients with lupus was negatively correlated with the disease activity of patients.Conclusion:The percentage of CD4~+CD25~+T cells in peripheral blood decreased,which may be a predictor of bacterial infection in patients with lupus,and it also may be an indicator of disease activity in lupus patients.