| Objective Meteorin is a secretory protein,which can promote astrocyte formation and induce axonal extension.Previous studies have shown that exogenous meteorin can help reduce the loss of neurons in the experimental model of Huntington disease,stroke,etc.Our study was to investigate the dynamic changes of meteorin protein after focal cerebral ischemia in rats and whether meteorin can reduce the death of neurons and astrocytes subjected to oxygen-glucose deprivation.Methods Male Spague-Dawley rats were randomly arranged into 6 groups,including sham group and the groups of 6h,12h,24h,3d and 7d after MCAO.Focal cerebral ischemia was induced by the use of a suture occlusion at the middle cerebral artery in the rats for 90min.Cortical brain tissue was isolated to measure the expression of meteorin protein by Western blot.Immunofluorescence was used to detect the cellar distribution of the meteorin protein in the brain section slides from the rats 7 days after MCAO.Cortical brain tissue was isolated from embryonic day 15-17 Spague-Dawley rat embryos and primary neuron culture was performed.And the primary astrocyte culture was performed with the cortical brain tissue from the Spague-Dawley rats born in 24 hours.The cells were divided into control,OGD,OGD+meteorin.CCK-8 and PI/Hoechst33342 staining were used to assess the cell viability.Results Compared with the sham,meteorin protein decreased after MCAO and reached the lowest point at day 3(P<0.05)and increased later.Meteorin was mainly expressed in neurons and astrocytes after focal cerebral ischemia.Exogenous meteorin helped the survival of astrocytes subjected to oxygen-glucose deprivation when compared with the OGD group(JP<0.05),while no significant survival of neurons was observed after the ischemic injury.Conclusion Meteorin protein is mainly expressed in neurons and astrocytes after the focal cerebral ischemia.Exogenous meteorin can help reduce the loss of astrocytes subjected to the oxygen-glucose deprivation injury. |
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