Effect Of Astaxanthin On Ochratoxin A-induced Lung Injury In Mice

As we all known,OTA is one of the main mycotoxins at present,and it causes great harm to the health of humans and animals.OTA can directly enter the lungs through breathing and cause lung damage,but there is currently no good prevention method for OTA-induced lung damage.ASTA is an internationally recognized strong antioxidant.It has a detoxification effect on mycotoxins,so it is speculated that ASTA may protect the lungs from OTA poisoning.This experiment is divided into three parts.The first two parts are to screen out the concentration of OTA and ASTA,and the last part is to find out the effect of ASTA on OTA-induced lung injury in mice.In the last part of the experiment,80 male C57 mice were selected(divided into 4 groups,20 in each group)to establish a subchronic OTA poisoning mouse model.By observing the changes of mental state,feed intake,body weight,organ ratio,lung pathological section,lung oxidation index and lung inflammatory index of mice in each group,the intervention effect of ASTA on lung injury in OTA-exposed mice was evaluated.The results showed:(1)OTA can cause symptoms such as poor mental state,rough coat,significantly increased urine output,decreased weight and feed intake,and lung swelling in mice;while ASTA can alleviate these symptoms,indicating that ASTA can alleviate the clinical symptoms caused by OTA.(2)Observation of HE and TUNEL slices through an optical microscope showed that compared with the Control group,the lungs of the mice in the OTA group were significantly congested,hemorrhage,exuded,alveolar ruptured,pulmonary interstitial widened,and extensive inflammatory cell infiltration,foamy macrophages aggregated and apoptotic cells increased.Compared with the OTA group,the mice in the OTA+ASTA group had milder lung symptoms.(3)Through the detection of oxidation indicators,it is found that OTA can increase the expression of Keap1 and the content of MDA in the lungs of mice(P<0.05;P<0.01),and reduce the expression of HO-1,Mn SOD and Nrf2 and the content of T-SOD and GSH in the lungs of mice(P<0.05;P<0.01);while ASTA can make these indicators tend to normal values.It shows that OTA exposure can induce oxidative damage to lungs of mice,while ASTA can inhibit OTA-induced oxidative damage in lungs of mice.(4)Through the detection of inflammatory indicators,it is found that OTA can increase the expression of My D88,NF-κB and TLR4 and the content of IL-1β and IL-6 in the lungs of mice(P<0.05;P<0.01);while ASTA can make these indicators tend to normal values.It shows that OTA exposure can induce inflammation to lungs of mice,while ASTA can interfere with the development of inflammation in lungs of mice induced by OTA.The above results confirm that OTA exposure can induce lung toxicity in mice;and ASTA can alleviate OTA-induced oxidative damage and inflammation in the lungs of mice through antioxidant and anti-inflammatory effects.