Gene Expression Profile Indicates Involvement Of Nitrogen And Auxin In Camellia Sinensis Lateral Roots Formation

Tea plant[Camellia sinensis(L.)O.Kuntze]is a perennial,evergreen,woody crop,belonging to theaceae Camellia.Tea plant is thermophilic and fears of low temperature.Tea is one of the most popular non-alcoholic beverages in the world.The popularity of tea is not only due to its rich flavor and taste,but also associated with many beneficial effects of human being.Tea plant is Nitrogen-like crop,applied nitrogen(N)fertilizer significantly increases the leaf yield.The macronutrient nitrogen(N)is a necessary component of multiple important compounds,such as DNA,amino acids,proteins,and some plant hormones.Plant roots can uptake inorganic N(nitrate and ammonium)and organic N(amino acids and peptides).However,in term of the plasticity,lateral roots are more sensitive to different nitrogen supplies.As we all know,N supply affects pant growth and development,and hormones regulates all stages of plant life cycle,so we can see many reports which expound the connections between nitrogen and hormones signaling pathways.Previous studies have reported that N-deficiency increases root auxin content,the auxin accumulation show a function in the effect of root-system architecture changes in answer to N availability.In order to further understand the function of auxin and nitrogen in LRs formation,we used transcriptome analysis of lateral roots formation of tea plant(Camellia sinensis)treated with low nitrogen concentration,high nitrogen concentration,indole-3-butyric acid(IBA)and N-1-naphthylphthalamic acid(NPA)using the RNA-seq.we harvested 136.13Gb Clean Data,with a minimum Q30 of 92.83%.RT-PCR analysis was used to confirm RNA-seq date.Integrating four treatments,296 common differentially expressed genes were annotated to nitrogen metabolism,plant hormone signal transduction,Glutathione metabolism and transcription factors,indicating potential regulation to lateral roots formation.Our study shows new insights into the mechanisms associated with nitrogen-auxin signaling pathways to regulate lateral roots formation.And we also provide the N utilization genes for future research.