Detection Of Initial Infection Sources Of Rice Bacterial Diseases In Main Rice Areas Of Jilin And Liaoning Province

In this study,there are 154 samples of rice seeds,paddy soil,paddy soil residues and leaves were collected from the main rice areas of Jilin and Liaoning.After pretreatment for the samples,PCR amplification technique was used to detect the pathogen of Xanthomonas oryzae pv.oryzae,Xanthomonas oryzae pv.oryzicola,Burkholderia glumae,Dickeya zeae.Identify seeds,soils,diseased bodies,and primary infection sources for bacterial diseases in the main rice areas of Jilin and Liaoning provinces.Among them,the results showed that the samples with positive bands were as follows: A total of 1 soil samples from Baicheng areas Jilin Province,6 seeds samples from Siping areas Jilin Province,and 6 seed samples from Da’an areas Jilin Province were used to carrying Xanthomonas oryzae pv.Oryzae;a total of 1 seed sample from Siping areas Jilin Province,1 seed samples from Da’an areas Jilin Province,and 2 samples of soil disease residues in Dandong areas Liaoning Province were used to carrying Xanthomonas oryzae pv.oryzicola;a total of 4 soil samples and 4 soil disease samples from Yanbian areas Jilin Province,3 soil samples and 3 soil disease samples and 1 seed sample from Jilin areas Jilin Province,1 seed samples from Da’an areas Jilin Province,4 seed samples from Siping areas Jilin Province were used to carrying rice bacterial blight pathogens Burkholderia glumae;a total of 15 seed samples from Da’an areas Jilin Province,9 seed samples from Siping areas Jilin Province,1 soil sample from Jilin areas Jilin Province,3 soil samplesand and 2 soil disease residues from Baicheng areas Jilin Province,3 soil samples from Changchun areas Jilin Province were used to carrying Dickeya zeae.Therefore,the soil、soil disease residues and seeds are the initial infection sources of bacterial diseases in the main rice areas of Jilin and Liaoning,and the degree of their infection is different,the types of pathogens in the initial infection sources from different regions are also different,in which the seed samples carried different germs at the same time.A pair of primers JLND-Xoc F: 5’-ATGTTTCCTTTGATGACG-3’and JLND-Xoc R:5’-TTACGCTGAGTAGCCTGT-3’were designed according to the sequence of Xanthomonas oryzae pv.oryzicola specific gene Av Rxo(AY395713.1)using Primer Premier 5.0 software,the test samples were tested,and when the specific band appeared 262 bp,the pathogen of Xanthomonas oryzae pv.oryzicola was determined.According to the known sequence of Burkholderia glumae specific gene 16 S r DNA(D87080),a pair of primers JLND-Bg F: 5’-GAATCCAACCAGACCCAC-3’ and JLND-Bg R: 5’-CGCTTGACCCTATAACGA-3’ were designed to detect the samples,when the specific band appeared 448 bp,the pathogen of Burkholderia glumae was determined.Studying and determining the source of initial infection of pathogenic bacteria,it has important significance in grasping the growth law and prevention of related bacterial diseases in forecasting prevention and theoretical practice.