Effects Of Low Temperature Treatment Of Musca Domestica Pupae On Parasitization And Reproduction Of Wolbachia Infected And Uninfected Nasonia Vitripennis

Housefly(Musca domestica)is one representative of health pests.They are the carriers of human and animal pathogens.Now we mainly rely on chemical insecticides to reduce the damage of houseflies,however,insecticide resistanceis is becoming more and more serious due tothe long-term use of chemical insecticides,so the prevention and control of the housefly is much more difficult.Therefore,more and more attention has been paid to the biological control methods.Nasonia vitripennis is one of the natural enemies,which can not only have a sustiainable and stable control effect,but also can prevent the harmful effect of pesticides on livestock,poultry,human beings and environment.In this thesis,we studied the reproductive effects of low temperature treated housefly pupae for infected and uninfected Wolbachia N.vitripennis,the results will provide theoretical and practical basis for factory production of N.vitripennis.The main results are as follows:1.Effect of different time of low temperature treatment on survival number of M.aomestica pupaeWith the decrease of temperature and the increase of treatment time,the survival number of M.aomestica pupae decreased obviously.When the M.aomestica pupae were treated at 4 C for 15 days,the survival rate of M.aomestica decreased rapidly,and it could hardly emerge after 30 days of treatment.2.Effects of low temperature treatment of housefly pupae on parasitization and reproduction of infected and uninfected Wolbachia N.vitripennisWith the decrease of temperature,there were significant differences in the average number of wasps,Female ratio,eclosion rate and prophase of adult between the infected and uninfected with Wolbachia N.vitripennis.Compared with the control group,the average number of the wasps,eclosion rate of the two kinds of N.vitripennis at 4℃ were higher than that of other low-temperature treatment temperature at 10 ℃ and-20 ℃,respectively,and the average number of wasps and eclosion rate of the Wrolbachia-infected N.vitripennis was higher than that of the N.vitripennis of the uninfected Wolbachia.3.Effects of low temperature treatment of M.domestica pupae at different times on the parasitization and reproduction of N.vitripennisWhen the pupae of M.domestic a were treated at low temperature,the average number of wasps,e cl o si on rate,Female ratio of the two types of N.vitripennis were decreased with the decrease of treatment temperature and time.Among them,the average number of wasps and eclosion rate of the Wblbachia-infected N.vitripennis was higher than that of Wrolbachia uninfected N.vitripennis.Within 15 days of low temperature treatment,the average number of wasps of N.vitripennus was more than 10 iundividuals,and the average number of wasps of Wolbachia-infected N.vitripenis was 14.50±0.42 and the eclosion rate was 43.75 ± 1.83%when the low temperature treatment was 4℃.Low-temperature treatment of M.domestica pupae can be used to factory production of N.vitripennis,and 4℃ cryopreservation within 15 days is the most suitable.4.Effect of low temperature treatment on the larval stage of N.vitripennis at different timeWith the decrease of low temperature and the increase of treatment time,the average number of wasps and eclosion rate showed a decreasing trend with the decrease of temperature during the larval stage(precocious stage)of N.vitripennis treated with low temperature,but there was no difference in Female ratio.Among them,the average number of wasp s and eclosion rate of th e Wolbachia-inf e cted N.vitripennis was hi gh er th an th at of th e Wolbachia-uninfe cted N.vitripennis.The larval stage(precocious stage)of N.vitripennis collected by low temperature treatment at different time,after treatment at 0℃ for 1 day,there was no wasps in the two types of N.vitripennis.The average number of wasps was more than 10 in 5 days and the eclosion rate was more than 50%under other temperature treatments.The average number of wasps was more than 10 in 20 days under 16 ℃ low temperature treatment,which could be used to expand the reproduction of N.vitripennis.After treatment at 16 ℃ for 5 days,the average number of wasps of Wolbachia-infected N.vitripenns was 19.63±0.94,and the eclosion rate was 70.00±2.58%With the exception of 0℃,the other low-temperature treatments could be used for factory production within 5 days of larval stage(precocious stage),and 16℃ low temperature treatment was the most suitable for 5 days.