| This study chose the medicinal plants of Asparagus cochinchinensis as the test material.On the basis of knowing the features of Asparagus’seed germination and the way to pretreat its seed,this study explored the conditions of Asparagus’callus induction and proliferation,induction and seedling,seedling and rooting,seedling and transplanting in a systematic way,thus preliminarily constructing the rapid propagation technology system of Asparagus.The main results were as follows:（1）In this part,the mature Asparagus seeds were taken as the test material to investigate how the different time of both concentrated sulfuric acid treatment and soaking in the warm water,different plant growth regulators and cultivation conditions influence the aseptic germination of asparagus seeds.The results showed that the seeds were soaked in concentrated sulfuric acid which treatment time was 4 to 8 minutes,and dipped 4 to 6 hours in warm water at 35 centigrade,then rapid immersed with 75%alcohol for 0.5 minutes plus 0.1%HgCl2 for 3 minutes,after that,the suitable medium of the seed germination was MS medium supplemented with 2.0 mg·L-1 6-BA and GA3 0.5 mg·L-1 and sugar 30 g·L-1,the germination rate was 83.49%.the germinated germ free seedlings after 15 d were suitable as explants for the rapid propagation system of Asparagus in vitro.（2）In this part,the pretreated Asparagus bud seedlings by aseptic germination were viewed as the test material,to conclude how the distinct plant growth regulator and concentration affect the callus induction and proliferation.The result showed that 6-BA and NAA had a significant impact on the callus producing and proliferating,the suitable medium of Asparagus’s callus induction was MS medium supplemented with 0.5 mg·L-1 NAA and 1.0mg·L-1 6-BA,with the callus induction of 86.67%,the callus was pale yellow and grew well.In the callus induction medium,it was found that the induced callus gradually turned green and easily differentiated into buds.It should be transferred to the callus proliferative medium,the medium for callus proliferation was MS medium supplemented with 2.0 mg·L-1 NAA and0.5 mg·L-1 6-BA,the callus proliferation of 9.71,the callus was pale yellow and had a good growth potential.（3）In this part,the small blocks of Asparagus cochinchinensis callus after proliferation were taken as materials,which were used to investigate the effects of different plant growth regulators kind or concentration and medium on the clusters producing.the result showed that the MS medium was the basic medium suitable for the induction of buds,the 6-BA,NAA and KT had a significant impact on the clusters producing,the orthogonal test with this three plant growth regulators shows that the optimal medium for cluster buds induction was MS medium containing 0.5 mg·L-1 6-BA and 0.1 mg·L-1 NAA and 0.2 mg·L-1 KT,with the induction of91.11%,the bud was green and flourish.（4）In this part,the induction of Asparagus cochinchinensis buds were taken as materials,which were used to investigate how IAA and 6-BA influence the buds’stretch and thicking.The results showed that the best hardening medium was MS medium supplemented with 1.0mg·L-1 IAA and 0.2 mg·L-1 6-BA,with the average plantlet height of 4.55 cm,and the test-tube plants were healthy.Then using stretched and healthy rootless seedlings as test material to figure out how the different concentrations of elements and NAA concentration affect seedlings on rooting.The combination trial results showed that the optimum medium for planted rooting was 1/2 MS medium containing 2.0 mg·L-1 NAA,with the rooting rate up to 84.44%,the root was strong and the root was well developed.（5）in this part,the tissue culture seedlings of Asparagus cochinchinensis were taken as the materials,which were used to investigate the effects of different seedling time and transplanting matrix on the survival of tissue culture seedlings.The result showed that the time of opening the bottle and the substrate of the transplanting had a significant effect on the survival of the tissue culture seedlings.The best time of opening the bottle was 3 d and the matrix formula ratio of peat soil:Perlite:the vermiculite was for 2:1:1,the survival rate of the transplant was 82.22%,the growth of the tissue culture seedling was green,and new leaf grew out.（6）The preliminary technology system constructed by this study which aimed in how the Asparagus seedlings rapid propagation in vitro was like this:selecting the mature and healthy seeds,treat them in the concentrated sulfuric acid for 4-8 min,after that soak them in the warm water which reach 35 centigrade for 4-6 h,then immerse them with 75%alcohol for0.5 minutes plus 0.1%HgCl2 for 3 minutes,and then inoculated in MS+6-BA 2 mg·L-1+GA3 0.5 mg·L-1+sugar 30 g·L-1.The medium was incubated in the medium for germination,and the medium was incubated in dark conditions for 3 d,then transferred to 12 h·d-1,and the light intensity was 2000 lx until the strong sterile shoots were grown,and the germinated sterile shoots were explants,inoculated with MS+NAA 0.5 mg·L-1+6-BA 1.0 mg·L-1culture.The callus was induced in the base formula,and the induced callus was transferred to MS+NAA 2.0 mg·L-1+6-BA 0.5 mg·L-1 to continue to proliferate,and the callus was cut into small blocks after the proliferation of the callus,and inoculated with MS+6-BA 0.5mg·L-1+NAA 0.1 mg·L-1.And the good sprouts were cut into 3 clusters of seedlings inoculated in the medium of MS+IAA 1.0 mg·L-1+6-BA 0.2 mg·L-1;until the bud grew into a non root of 5 cm,it was inoculated into the root culture medium of 1/2 MS+NAA 2.0mg·L-1.Peat soil:Perlite:vermiculite is a matrix of 2:1:1,and its seedling yield can reach more than 80%. |
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