| Plants have evolved a lot of respond mechanism to respond to the changing environment in the process of evolution,including induced heat shock proteins.DnaJ protein,as a kind of heat shock protein,play an important role in many biological and abiotic stress.DnaJ protein is also called heat shock protein 40(HSP40),and is consist of four conserved structures: a J-domain,a proximal G/F-domain,a distal zinc finger(CxxC xGxG)domain,and followed by less conserved C-terminal sequences.Up to now,many DnaJ proteins in organisms were identified,such as Arabidopsis thaliana(89),yeast(22)and human(41).Despite ongoing efforts to characterize the members of J-protein from other organisms,none of its from pepper has been identified at the genomic level.Fortunately,the pepper whole genomic sequences were completely available,which provided an opportunity for identifying candidate DnaJ protein genes at the genomic level.In the present work,we have identified 76 DnaJ proteins used bioinformatics methods and were named as CaDnaJ01 to Ca DnaJ76.According to the presence of the complete three domains(J-domain,zinc finger domain,and uncharacterized C-terminal domain),they were classified into five groups(A,B,C,D and E),including 9,8,53,1 and 5 members,respectively.O n the basis of sequence homology,they can be divided into seven subfamily(I,II,III,IV,V,VI and VII).All these CaDnaJ genes(9 members in group A,8 members in group B,53 members in group C,1 members in group D and 5 members in group E)in pepper were uneven distributed on 12 chromosomes.One tandem duplication event and two segmental duplication events were detected.The results suggested that these duplication events made contributions to pepper CaDnaJ genes expansion.The upstream regions(1.5kb)of the CaDnaJ sequences were searched for regulating factor on different stress conditions.The result showed that multiple of stress related cis-element were found in the promoter region of these CaDnaJ genes.It was indicated that Ca DnaJs may involved in the process of plant respond to multiple stresses.To obtain more insights into the expression profiles of Ca DnaJ genes in different tissues,RNA-seq data were acquired from leaf,root,stem and different stage of pericarp.Based on RNA-seq,we found that 49(61.84%)CaDnaJs have expressed in at least one tissues,It was implied that CaDnaJ genes may played important role in the growth and development of pepper.To gain more insight into the role of Ca DnaJ genes under different condition,expression profiles of Ca DnaJ genes in pepper based on qRT-PCR technique were performed.In this article,there are eight genes(CaDnaJ25,47,56 in subfamily III,CaDnaJ10,40,74 in subfamily IV,CaDnaJ70 in subfamily V,CaDnaJ46 in subfamily VII)have obvious difference expressed in with spicy and not spicy chili significant difference,indicating that these genes may be associated with the formation of capsaicin.Also,the eight gene expression is regulated by the adversity stress.In different gene expression under adversity stress varying degrees of changes have taken place.As is known to all,there are many genes such as drought,high salt and low temperature under adversity stress adjustment,at the same time regulation by plant hormones,the structure of this study also confirms this.Under four different hormone treatment,related to the capsaicin synthesis and the expression of 8 genes is regulated by the adversity stress varying degrees of changes have taken place in the same,this suggests that the hormone involved in the process of capsicum adversity stress response.These results will provide further analysis CaDna J protein by members of the functional composite foundation,at the same time also have important significance to study the synthesis of capsaicin. |
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