Comparative Analysis Of DNA Methylation In Red And White Muscle Tissue Of Takifugu Rubripes

Takifugu rubripes is a widely used,high economic value and nutrient-rich breeding model organism.There have been extensive studies on the physical and chemical environment,culture modes,and molecular levels that affect the growth rate.With the deepening of the research,it is known as knocking open the transcriptomics and genomic link-DNA methylation has attracted more and more scholars’ interest.In this study,the red muscle and white muscle tissues were used as experimental materials for the study of the Takifugu rubripes.DNA methylation,one of the epigenetics inheritance phenomena,was analyzed by genome-wide bisulfite sequencing analysis.By means of bioinformatics analysis,DNA methylation modification information affecting the growth and development of the muscle tissue of Takifugu rubripes was obtained.In-depth analysis of methylation modification of muscle growth and development related genes,and preliminary at the level of signaling pathway,discussed the impact of DNA methylation modification.By comparing the results of methylation data of red and white muscles,we found: myogenic regulatory factors(MRFs),boxed transcription factor family(Pax),and somatostatin 2 gene(Myostatin 2),there are significant differences in methylation modifications.Among them,methylation modifications of red muscle Myostatin2,Myf5,MyoD,and Pax3 inhibit red muscle atrophy and promote the formation of red muscle;Pax7 and MyoG that occur in white muscle increase muscle fiber formation and fat content,and promote white muscle growth.With weight gain,these results may be interpreted as an explanation of both muscle differentiation and growth.Examine the level of methylation modification of related signal factors in mitogen-activated protein kinase signaling(MAPK)and GH-IGFs signaling pathways that affect muscle growth and differentiation,and obtain a large number of possible effects on myocyte proliferation and myocyte differentiation.The DNA methylation modification of the anchoring gene and the muscle cell nutrient ratio.Among them,MAX(MYC associated factor X),protein tyrosine phosphatase(ptp),mitogen-activated protein kinase-activated protein kinase 5(PRAK)and other regulatory factors In the white muscle,the level of methylation modification is high,inhibiting the increase of white muscle fibers,reducing the protein content,and improving the muscle mass of the red muscle to become an excellent exercise organ.Insulin-like growth factor receptor(igflr1)and growth hormone receptor(GHR)are more methylated in red muscle than white muscle,which contributes to the increase of myogenic fibers and promotes white muscle fast growth.Through WGBS sequencing technology and bioinformatics analysis methods,this study provides a large amount of data for the epigenetic study of the growth and development of Takifugu rubripes,and it also lays a foundation for DNA methylation research of Takifugu rubripes muscle tissue.