| Because of its good peeling performance and high AsA content,Actinidia eriantha Benth has attracted the attention of researchers.In this study,145 wild A.eriantha germplasm resources in Magushan Nature Reserve of Nancheng County,Fuzhou City,Jiangxi Province,were used to analyze the variation and correlation of wild A.eriantha germplasm.The methods of principal component analysis and cluster analysis were used to analyze and discuss the main internal quality indexes of mature fruit,which could provide material basis and evaluation reference for breeding excellent new varieties of Actinidia.The genetic diversity of wild A.eriantha germplasm was analyzed by using SSR molecular marker technique,and the genetic diversity at molecular level and the difference and relationship between wild kiwifruit germplasm were revealed.It can provide theoretical basis for the sustainable development and utilization of germplasm resources.On the basis of the above,the correlation between the natural variation of main endoplasmic indexes and SSR markers of wild A.eriantha fruit was detected by using the method of correlation analysis.It can provide theoretical basis for exploring the superior alleles and quality genetic improvement of wild A.eriantha.The main findings were as follows:（1）The 7 main internal quality indexes of 145 wild A.eriantha were all varied.The coefficients of variation from big to small were soluble sugar content,carotenoid content,chlorophyll content,titratable acid content,AsA content,DM content and SSC,which respectively were 39.19%,35.17%,34.30%,20.25%,19.48%,19.04%and 18.70%.And the average value is 26.59%.The corresponding variation ranges of each index were1.39%～12.87%,0.3～2.79mg.g-100,1.15～10.26mg.g-1,0.78%～2.51%,3.53～9.40mg.g-1,6.64%～23.08%and 6%～17%.The correlation between 7 endoplasmic indexes of wild A.eriantha was discussed through correlation analysis.Principal component analysis（PCA）transformed 7 original endoplasmic indexes of wild A.eriantha into 4 new,comprehensive and independent principal components.Through the contribution rate of 4 principal components and the corresponding membership function value,all samples were comprehensively graded by weighted method,and 16 excellent wild kiwifruit germplasm were selected.Based on cluster analysis,145 wild A.eriantha resources were divided into 6groups with genetic distance of 1.486.（2）A total of 224 alleles were detected by 40 pairs of SSR primers in genetic diversity analysis of Wild A.eriantha Germplasm Resources through SSR markers.The number of alleles detected by each pair of primers ranged from 3 to 13.An average of 5.6 alleles were detected by per pair of primers.The average number of effective alleles is 2.83,Shannon’s information index is 1.12,Nei’s gene diversity index is 0.566,the expected heterozygosity is 0.57 and the apparent heterozygosity is 0.54.At the genetic distance of 0.099,the wild A.eriantha germplasm source was divided into 13 groups in UPGMA cluster analysis.（3）40 pairs of SSR markers covering the whole genome were used to analyze the population structure and linkage disequilibrium（LD）of the tested materials.And the GLM model in Tassel 2.1 software was used to analyze the correlation between the SSR markers and the main endoplasmic indexes of wild A.eriantha.Population structure analysis divided the tested materials into 3 subgroups.After conditional filtration of gene frequency of 0.05,there were significant linkage disequilibrium in 35 SSR polymorphism markers.Further association analysis showed that,under the condition of P<0.05,8 markers（UDK96-035、UDK96-039、UDK96-040、A193、FOR11、FOR22、ST-Acd07、UTH03-04）were found to be associated with 5 quality traits,namely soluble sugar,titratable acid,chlorophyll,DM and SSC.The primer A193 was significantly correlated with soluble sugar under the condition of P<0.01. |
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