Development Of Molecular Markers For Potato Tuber Protein And Expression Analysis Of Related Genes

The protein content of potato tubers is an important quality traits,and the current research on the genetic mechanism is relatively scarce.In this study,genetic segmentation of tuber protein content was controlled by combining high-throughput simplified genome sequencing with BSA pool construction in the tetraploid potato population.Finding a genetic section on the protein content of potato tubers on chromosome 8 to develop a molecular marker related to potato tuber protein content.The markers developed were tested and validated by tetraploid potato varieties and offspring of tetraploid potato separation populations.Ultimately marker protein content of potato tubers with tightly linked molecules may be assisted breeding;By analyzing the expression levels of related genes on the potato protein synthesis pathway in different varieties,the expression patterns of genes in different varieties were compared to provide reference for the exploration of potato protein synthesis.The main findings are as follows:1.For two consecutive years,172 progeny of the cross combinations prepared from the Atlantic(parental)and Dingshu 1(father)were used to determine tuber white matter content,and 74 high protein content offsprings,42 low protein content offspring and 57 offspring protein content.were obtained2.A DNA pool was constructed with 25 extremely high protein content materials and 25 extremely low protein content materials in separate populations.Sequential SNP loci were screened within the whole genome of the potato in combination with high-throughput simplified genome sequencing to obtain extreme mixing Pool specific difference labels.3.According to the screen of genetic diversity of tuber protein content,a total of 34 pairs of primers were designed and 3 specific molecular markers SCAR8-107,CAPS8-101,and CAPS8-110 were closely linked to the protein content of potato tubers.These three markers reached 90.51% and 72.97% respectively in the detection results of 116 offspring isolates and 54 tetraploid potato varieties.The results showed that the molecular markers developed in this experiment could be applied to the preliminary screening of protein content in potato marker-assisted breeding.4.Based on four genes involved in the synthesis and degradation of potato protein and six potato cultivars,the expression level of each gene in different potato cultivars was determined and analyzed by RT-qPCR.The gene expression patterns of Class I patatin gene,amino acid transporter gene and cationic amino acid transporter gene involved in potato protein synthesis were consistent throughout the reproductive period.The expression of proteasomal β1 subunits involved in the specific degradation of potato protein was consistent in potato varieties,and was significantly upregulated in the late growth stage.And the correlation analysis showed that Class I patatin gene and amino acid transporter gene showed a significant positive correlation with potato protein,indicating that Class I patatin gene and amino acid transporter gene are important regulatory genes for the formation of potato protein.