Correlation Investigation Between Histone Modification H3K4me3 And H3K27me3 And Meiotic Homeologous Recombination In Rice Tetraploids

Rice is one of the most important crops in the world,but with the global population growth,climate and environment deterioration,the rice yield can not achieve substantial growth,leading to a potential food crisis.In the process of plant evolution,polyploidy events occur frequently[1][2][3],according to statistics,in the cultivation of crops which is as high as 75%[4][5].And according to the research,which shows that the polyploidy and combination of heterologous genome are the overall trend of plant evolution.And the content of polyploidy body proteins,amino acids and carbohydrate,vitamins,and other nutrients and alkaloid metabolites have increased,which will undoubtedly bring a new hope for the potential food crisis,provides a new idea for increasing rice production.At present,the research of polyploidy rice has become the focus of plant research.The researchers expect the polyploidy rice for the breeding of high yield,high quality and multi resistance unified ’super rice’ variety provides a good theoretical basis.Homologous recombination is an important means of genomic variation and an indispensable step in the process of biological evolution.At present scientists to many organisms(Human,Mouse,Drosophila,Yeast and Arabidopsis)homologous recombination were reported,but the reports of certain sections or confined to the genome,or the whole group,while the number of SNP used in Marker is very limited and we don’t make a comprehensive and effective judgment on homologous recombination events.Previous studies have shown that homologous recombination is associated with many factors such as the number of DSB,histone modifications,Chromatin structure,and so on.The aim of this study was to analyze the correlation between homologous recombination and histone modification of H3K4me3 and H2K27me3 in Rice by combining the next generations sequencing and Ch IP-seq results.We analyzed a total of 6 tetraploid rice(WTJ7(NN99),WTJ12(NN99),WTJ14(NN99),WTJ24(99NN),WTJ34(99NN),WTJ37(99NN))materials,from the whole genome and identification of recombination sites,found that the average material about 121.33 recombination sites at the same time,the distribution of recombined sites on the genome has certain characteristics,Chromosome is longer,the higher the probability of occurrence of recombination.Then we analyzed the correlation between H3K4me3 and H3K27me3 and recombination sites from three viewpoints of Peak Number、Peak Coverage and Read Density.Three angles to get the same conclusion that we find that the two epigenetic markers(H3K4me3 and H3K27me3)were not significantly enriched in recombination sites,namely it was not significantly correlative between recombination sites and histone modification H3K4me3 and H3K27me3.