Preparation Of Functionalized Bamboo Charcoal Nanoparticles And For Its NIR-stimulate Drug Release Properties

This chapter introduces traditional methods of cancer treatment,including chemotherapy,surgery,and radiation therapy.These traditional treatment methods have certain side effects,so cancer treatment methods with small side effects need to be developed.One of the current methods of cancer treatment is photothermal therapy(PTT),which is based on a photothermal agent that converts near-infrared light into heat to kill cancer cells.Photothermal therapy is based on the difference in photosensitivity between cancer cells and normal cells,using heat to kill cancer cells,so photothermal therapy has fewer side effects than traditional treatments.Chapter 1: This chapter introduces drug delivery systems,ways to induce drug release,and methods of treating tumors with near-infrared light-induced photothermal agents.The research progress of carbon-based nano drug carriers in cancer treatment was introduced in detail.Chapter 2: This chapter introduces bamboo charcoal nanoparticles(BCNPs),which was synthesized by the commercial bamboo charcoal powder treated with physical grinding and ultrasonication.Doxorubicin(DOX),as a model drug,was used to adsorb on the surface of BCNPs via ?-? stacking.After drug loading,polydopamine was modified on the surface of BCNPs by physical adsorption to form the drug carrier system,which was developed as a thermo-sensitive drug delivery carrier.The structures,potentials and morphologies of BCNPs and BCNPs@PDA were characterized by infrared spectroscopy,transmission electron microscopy,ultraviolet-visible spectroscopy and zeta potential.Infrared spectroscopy and UV-visible spectroscopy analysis confirmed that PDA was successfully modified onto the surface of BCNPs.The particle size distribution of BCNPs and BCNPs@PDA,which was observed by transmission electron microscopy are(42±10)nm and(50±10 nm),respectively.Fluorescence spectroscopy was used to study the performance of drug loading on the BCNPs.The results showed that the drug loading on the BCNPs accorded with the Freundlich multilayer adsorption mode.Analysis of in vitro release of DOX showed that the amount of drug released under the near-infrared light was greater than that in the absence of near-infrared light.Chapter 3: A novel drug carrier is engineered by grafting folic acid(FA)-chitosan(CS)composite which is garnered by amide reaction to the surface of bamboo charcoal nanoparticles(BCNPs)via static electrostatic adsorption interaction self-assembly.Doxorubicin(DOX),as a model drug,was used to adsorb on the surface of BCNPs via ?-? stacking.The morphology and structure of BCNPs and BCNPs@CS-FA were characterized by transmission electron microscopy and infrared spectroscopy.FA-CS layer ameliorates the targeted intracellular delivery performance due to the overexpressing the folate receptor in lung cancer A549 cells.In vitro cell toxicity experiment indicates the cell inhibition is co-regulated by NIR based photothermal therapy and enhanced drug release.The strategy of combining the stimuli-responsive of nanocarriers with targeting folic acid will offer new directions in on-demand and sustained drug release for chemotherapy.Chapter 4: The folic acid and bovine serum albumin(FA-BSA)polymer are synthesized by an amide reaction,and then the polymer is modified on the surface of the bamboo charcoal nanomaterial by physical adsorption.The morphology and structure of BCNPs and BCNPs @ BSA-FA were characterized by transmission electron microscopy,infrared spectroscopy and UV-visible spectroscopy.The results showed that the drug carrier was successfully synthesized,which laid a foundation for the further study of BCNPs @ BSA-FA as a drug carrier.Chapter 5: The chapter summarized the modification of BCNPs surface and the loading behavior of anticancer drugs in drug delivery systems.The application of BCNPs as drug carriers has great application prospects.