Study On Functional Components Analysis And Hairy Root Induction Of Rosa Rugosa

Rosa rugosa Thunb.is not only a garden plant with excellent ornamental and economic value,but also a traditional medicine food homology plant in our country,which flowers can be extracted rose essential oil and rose tea.Its roots can be used as medicine,with high medicinal and health-care values.Moreover,diversification of products,can fully be popular with the market.However,different rose products have different materials and processing methods,resulting in the functional components are also very different.In this study,the main varieties of roses in China were used as test materials,and the functional components and antioxidant capacity of rose tea made under different opening degrees and different drying methods were tested and compared.A technical system for the extraction and detection of Rosamultin,a functional component with HIV resistance in rose roots,was established,and its contents in different germplasm materials were compared.A technical system for induction of R.rugosa hairy roots has been initially established,which could be used as an expanded reaction vessel for metabolites such as Rosamultin and establish base for biosynthesis of functional components of R.rugosa.The main research results are as follows:1.We chosen water as the solvent of hot reflux extraction with different opening degrees and different drying processes of rose tea,which detected the antioxidant capacity of extracts and active components.The results showed that in negative ion mode,nine components were isolated and confirmed by reference substance.The polyphenols were paeonol and gallic acid,respectively.The remaining flavonoids were proanthocyanidin B3,epicatechin,dihydroquercetin,rutin,quercetin-3-O-glucoside,guavarin and quercetin.The antioxidant capacity of Rosa rugosa may be related to these active components and their content.The results showed that proanthocyanidin B3 and epicatechin were the highest in half-opening stage,dihydroquercetin,gallic acid and paeonol were the highest in budding stage,quercetin-3-O-glucoside,guavarin,rutin and quercetin were the highest in full opening stage.The contents of gallic acid,rutin,proanthocyanidin B3,quercetin-3-O-glucoside,epicatechin,guavarin and quercitrin in rose tea prepared by vacuum freeze-drying were higher than those of rose tea prepared by low-temperature drying.While dihydroquercetin and paeonol are just opposite.The total antioxidant capacity,anti-superoxide anion free radical capacity and hydroxyl free radical capacity were the stronger in budding stage.The total antioxidant capacity,superoxide anion scavenging capacity and hydroxyl free radical inhibiting capacity of the water extract of rose tea prepared by low-temperature drying process were significantly lower than that of the water extract prepared by vacuum freeze-drying process.All of the above components have antioxidant and anti-inflammatory capabilities,which provide theoretical basis for further research on different pharmacological effects of Rosa rugosa.2.A technical system for the extraction and content detection of Rosamultin in Rosa rugosa roots was established,and the difference of the content of Rosamultin in the roots of 11 species of rose was compared.The results showed that the highest concentration of Rosamultin was 20.233?g/mL in Rosa davurica Pall,and that in Rosa dahurica Pall and Rosa corymbulosa rolfe were also higher.The content of Rosamultin in wild rose roots in Hunchun was significantly higher than that in Weihai,which indicated that the content of Rosamultin was significantly different among the roots of different Rosa rugosa species.As one of the most common plants in China,Rosa davurica Pall.has a great potential in exploitation and application.3.Through screening the gradient conditions of five factors including pre-culture time,co-culture time,bacterial solution concentration,infection time and medium type,the optimum conditions were established.We used the leaves of R.rugosa 'Zizhi' seedlings as explants,1/2 B5 as the medium.After two days,we used Agrobacterium rhizogenes ATCC15834 to infect the leaves,OD600 value of bacterial solution was 0.6 and the infection time was 15 minutes.After 3 days of co-culture,the highest induction rate could reach about 32.86%.The infection time and co-culture time should not be too long,otherwise the strain at the wound will be enriched,leading to the easy browning of the leaves during the later sterilization process,which reducing the induction rate of hairy root.1/2 B5 liquid medium is conducive to the growth of hairy root of rose.PCR results of hairy root confirmed that the T-DNA of Ri plasmid has been integrated into the genome of hairy root of rose,indicating the acquisition of hairy root of rose.As a reaction vessel of secondary metabolites,hairy roots can provide a basis for industrial production of rose products.