Concentration Of Sugars From The Enzymatic Hydrolysate Of Lignocellulose By Nanofiltration

Removal of fermentation inhibitors and concentration of monosaccharides are essential to bioconversion of lignocellulosic biomass to bioethanol. Nanofiltration (NF) is a promising membrane separation technology due to its low energy consumption and unique separation properties. This work studied the concentration of hydrolyzates obtained from enzymatic hydrolysate of cornstalk, miscanthus and reed using nanofiltration (NF).The optimum conditions of concentration sugar were determined with the single factor and the response surface method.The results are as follows:The optimized processing technology was obtained by single factor experiment and the response surface method. The parameters of processing technique were determined and the optimal process condition was recommended (22 min,17 bar,5L/min).On this condition, glucose and xylose concentrate up to 53.19 g/L and 23.01 g/L,respectively.Enzymatic hydrolysate of cornstalk(glucose and xylose content are 8.3 g/L and 3.65 g/L respectively),miscanthus(glucose and xylose content are 5.64 g/L and 2.99 g/L respectively) and reed (glucose and xylose content are 6.28 g/L and 3.48 g/L respectively)are compounded to concentrate by using nanofiltration membrane, hydrolysis condition 22 min,17 bar,5L/min.Concentrated cornstalk liquid content glucose and xylose 50.20 g/L?21.27 g/L,respectively;Concentrated miscanthus liquid content glucose and xylose 34.65 g/L?18.72 g/L,respectively; Concentrated reed liquid content glucose and xylose,38.15 g/L?20.88 g/L,respectively. Fusarium oxysporum was vaccinated in cornstalk, miscanthus and reed concentration liquid,producing 21.32 g/L,8.02 g/L,8.43 g/L of ethanol, and get 45.4,50.1,28.0 times. Using nanofitration concentrate cellulose hydrolyzates can significantly improve the effect of ethanol fermentation.