Membranes Of Composite Graphene Oxide/Chitosan/Hydroxyapatite For Guided Bone Regeneration

In recent years,research on guided bone regeneration(GBR)is still ongoing,with evidence mainly from preclinical studies.GBR characteristics are required to provide the maximum membranes function and mechanical support to the tissue during bone formation.The researches about graphene and graphene oxide(GO)based composites have been active because of their excellent performance and they have been applied in biomedical and regenerative engineering.Chitosan(CS)as a kind of natural amino polysaccharide,due to the existence of active groups also has excellent ability to be functionalized and compounded with other materials.Hydroxyapatite(HAP)is the main inorganic component in bone.In this study,GO/CS hybrid membranes were fabricated using a one-step vacuum filtration method and synthesised GO/CS/HAP composite membranes by biomimetic mineralization and their potential applications in GBR were investigated in terms of their in vitro cellular activity and in vivo bone regeneration ability.The morphology and the surface hydrophilicity of the membranes were characterized by SEM,FIRT,EDS and water contact angle.Osteoblastic cells were observed to adhere well,proliferation activity and alkaline phosphatase activity on the hybrid membrane to a level higher than that observed on the GO and GO/CS membranes.In vivo study in a SD IGS Rats bone defect model demonstrated significantly enhanced bone regeneration using the hybrid membrane compared GO and GO/CS membranes.The results of our study showed that the GO/CS/HAP composite membranes could be a potential materials for GBR.