The Role Of LncRNA 9130024F11Rik In Mouse Brain Development

Recent years,a study showed that only one-fifth of the human genome was associated with protein coding,while the rest were mostly non-coding RNA.One type of non-coding RNA with a length greater than 200 nucleotide(nt),also known as LncRNA,has attracted the attention of many researchers because of its function of regulating gene expression.At the same time,with the “brain plan” of various countries and regions being proposed,relevant research on brain development process has emerged endlessly.A large number of existing studies have shown that a variety of LncRNAs are involved in brain development,including neuronal differentiation,migration,establishment of synapses,and regeneration of nerve cells.These functions are also closely related to the brain development.The brain development is closely regulated by a variety of factors.Among them,SATB2 protein plays a very important role in brain development as a transcription factor that binds to the attachment region of the nuclear matrix.The SATB2 protein extends axons to the corpus callosum,ensuring that the nerve impulse signals between the hemispheres are coordinated.At the same time,SATB2 protein is also involved in the formation of taste and long-term memory formation.All of them indicates that SATB2 plays an important role in brain development.In the previous survey,our team found that there is a LncRNA named 9130024F11 Rik in the opposite direction upstream of the SATB2 gene in mice,which is immediately upstream of SATB2,with some of the sequences overlapped.Previous studies in Allen Brain Atlas have shown that this LncRNA is specifically expressed in the brain,cerebellum and intestine of mice.In order to explore whether this LncRNA plays a role in the mice brain development,a 930024F11 Rik knockout mouse strain was designed and constructed.Finally,we successfully obtained the knockout mice of this LncRNA,and after testing,we believe that the construction of the knockout mouse was successful.Afterwards,we tested the knockout mice' morphology and gene expression levels.The final test results showed that 9130024F11 Rik and Satb2 gene transcription levels in the LncRNA homozygous knockout mice were lower than wild type and heterozygous knockout mice.Also during the Postnatal 14(P14)period,the change in brain size of the knock-out mice was successfully detected.At the same time,by observing the whole brain longitudinal section of the P14 period,we unexpectedly found that in the P14 period homozygous knockout mouse cerebellum,the outer granular layer is almost non-existent.This result indicates that there is a certain abnormality in the cerebellum development of the knockout mouse.Since there is no previous report on the expression of SATB2 protein in the cerebellum of mice,more experiments are needed to explore this result.After the research of this subject,we successfully constructed a 9130024F11 Rik knockout mice strain.In the homozygous knockout mice,the subject successfully observed that the mouse brain size became larger on the 14 th day after birth.Through sectioning and Nissl staining,the subject also found that there are abnormalities in the development process in the knockout mouse cerebellum.Then,by detecting RNA transcription levels,this subject also found that the expression levels of 9130024F11 Rik and Satb2 were decreased in knockout mice.Combining these observed phenomena,we believe that LncRNA 9130024F11 Rik plays a role in mice brain development.