Further Study Of The Mechanism By Which Valsartan Inhibits Peritoneal Fibrosis

Objective:To observe the effect of valsartan on the peritoneal structure and ultrafiltration volume andthe expression of TGF-β1,p-Smad2 / 3 and Smad7 in peritoneal tissue of rats with peritoneal fibrosis.To explore the inhibitory effect of valsartan on peritoneal fibrosis rat abdominal dialysis and its mechanism and to help identify new targets for the treatment of peritoneal fibrosis.Methods:The model of rat peritoneal fibrosis ware established by intraperitoneal injection of 1.0ml /(100 g · d)of glucose chlorhexidine at a concentration of 0.1%.A total of 30 male SD rats(clean grade)were randomly divided into control group,model group(peritoneal fibrosis group)and experimental group(model + valsartan group),10 rats in each group.Three groups of rats were given free water,alternating every 12 hours of circadian rhythm.Adoptive feeding rats before the start of the experiment for on week.Rats in the control group were given intraperitoneal injection of normal saline 1.0ml/(100g · d).Rats in the model group were given 0.1% glucose chlorhexidine intraperitoneal injection of 1.0 ml /(100 g · d).Rats in the experimental group were given intraperitoneal injection of 0.1% Glucose Chlorhexidine 1.0ml /(100g · d)to establish rat peritoneal dialysis peritoneal fibrosis model,valsartan 2.0mg /(kg · d)dissolved in0.5m L 0.9% saline daily intraperitoneal injection at the same time.Select the right lower abdomen of the rats for injection.The above operation for a total of 14 days.After 14 days,the peritoneal ultrafiltration volume was measured.After the measurement of peritoneal ultrafiltration volume,the rats were sacrificed and the peritoneal tissue was obtained.The histopathological changes of the peritoneal tissueswere observed by HE staining.The expression of TGF-β1in the peritoneal tissuewas detected byimmunohistochemistry.Western blot was used to detect the expression of p-Smad2 / 3and Smad7 protein in the peritoneal tissues.All the experimental data were analyzed by SPSS 9.0 statistical software.Metrological data were expressed by mean addition an subtraction standard deviation.Comparison between groups using single factor analysis of variance.P< 0.05 was considered statistically significant.Results:The peritoneal dialysis rat model with peritoneal fibrosis were successfully replicated by 0.1% glucose chlorhexidine intraperitoneal injection.Compared with the control group,the rats in the model group and the experimental group had lower peritoneal ultrafiltration volume and peritoneal thickening.The expression of TGF-β1,pSmad2 / 3 and Smad7 in the peritoneal tissue was significantly increased.The peritoneal ultrafiltration of the experimental group was significantly improved compared with the model group,and peritoneal thinning and structural improvement.The expression of TGF-β1,p-Smad2 / 3 in the peritoneum of the experimental group suppressed.The expression of inhibitory protein Smad7 was also inhibited.Conclusion:(1)Valsartan can improve the rat peritoneal structure and ultrafiltration function to a certain exent and inhibit the process of peritoneal fibrosis.The inhibitory effect is achieved by down-regulating the expression of the TGF-β/Smad pathway.(2)After administration of valsartan to the development of peritoneal fibrosis.The expression of TGF-β1 and p-Smad2 / 3 was decreased in the peritoneal tissues,while the expression of the inhibitory signal protein Smad7 was also inhibited.