| The incidence of pulmonary interstitial fibrosis is increasing due to the extensive use of chemical drugs and the increasing environmental pollution in recent years.In this paper,pulmonary fibrosis from the theory of lung atrophy,lung paralysis,lung theory were discussed,and further explore the pulmonary network and pulmonary fibrosis pathogenesis,to a more comprehensive understanding of the ancient understanding of lung disease And treatment experience,in order to guide Chinese medicine better treatment of pulmonary fibrosis.On the other hand,the effects of the active ingredients of astragalus angustifolia angelica on the neonatal growth of lung cells were studied.The optimal regimen of pulmonary fibrosis was found by different dosing regimen,and the theory Mechanism for the clinical prevention and treatment of IPF to provide experimental basis.Theory researchTheoretical discussion:First,from the perspective of evolution and the three embryo point of view,the emergence of the lungs in the period of higher vertebrate,mainly by the endoderm and ectoderm of the main;meridian is in accordance with the order of the spleen and stomach order,Evolved,a strong evidence of the lungs fur,from the coke also follow the appetite of the scientific.From the philological point of view,the lungs as early as the "Nei Jing" that there,and scattered in the "clinical guide medical case","medical door law" and other works,its function through the camp health,exotic,infiltration irrigation Blood,in the function of the lung plays a pivotal role.Pulmonary heart meridian connected,blood dependent,if the heart can be caused by lungs and lungs,so the author of the pulmonary fibrosis of the machine for the heart of burning lungs,Yin deficiency and blood stasis,for the prevention and treatment of pulmonary fibrosis to provide a new theory Thinking.Theory Discussion II:Chinese medicine does not correspond to the name of the pulmonary fibrosis,ancient physicians to lung impotence,lung paralysis of the.In this paper,the relationship between pulmonary fibrosis and pulmonary impotence,pulmonary paralysis and pulmonary network in terms of disease name,pathogenesis and treatment are expounded from the perspective of ancient Chinese literature,such as "Yellow Emperor","Treatise on Febrile Diseases" and "Jingyue Quan".The Experimental researchAnimal model and administration method:105 cases of SPF grade male ICR mice,weighing 18-20g,derived from Beijing Si Beifu Experimental Animal Technology Co.,Ltd.[Issue No.11401500007203,license number SCXK(Beijing)2011-0004]The The experimental animals were housed in dark,quiet and warm clean animals,fed for 3 days to allow them to cope with the environment.During the experiment,the mice were free to eat and drink.Were randomly divided into 7 groups,namely,normal group,model group and 5 astragalus angelica constituents to optimize the treatment group(ie,optimization group 1,optimization group 2,optimization group 3,optimization group 4,optimization group 5),Each group number 1-15.,Observe the survival status of mice and death,21 days after taking lung tissue and serum reserve.Perform the following experiment.Experiment 1:Effects of Optimized Formulas of Effective Components of Astragalus Angelica on Survival State and Morphology of Pulmonary Fibrosis in Mice.The changes of body weight were observed and the mortality curve was observed.The morphological changes of lung tissue were observed by HE staining and aniline blue staining.The results showed that the optimal formulation of Astragalus angustifolia angelica was helpful to improve the survival status and survival rate of mice,which could reduce the mortality of mice,and the effect of optimized one side and optimized 5 was obvious.Pathological morphology showed that compared with the model group,the treatment group had different degrees of improvement,and the alveolar hyperplasia of c group improved significantly,alveolar structure has been basically restored,inflammatory exudation was significantly reduced,indicating significant treatment;e,G group of pulmonary fibrosis lesions also improved.Experiment 2:Effects of Optimized Formulas of Effective Components of Astragalus Angelica on Hydroxyproline and Collagen in Pulmonary Fibrosis Mice.The levels of hydroxyproline in lung tissue homogenate of IPF mice were determined by alkaline hydrolysis.The expression levels of TGF-β,VEGF,Ⅰ,Ⅲ,Ⅳ and Ⅴ collagen were detected by RT-PCR.The results of HYP showed that the model group>excellent group 2>excellent group 5>excellent group 4>excellent group 1>excellent group 3>normal group,from the statistical results of excellent group 3 and excellent 1 Group was more significant.Compared with the normal group,the expression levels of TGF-β and VEGF were increased in the model group,and the expression levels of type Ⅰ,Ⅲ,Ⅳ and Ⅴ collagen were increased,May be one of the causes of the final increase in collagen content in the lungs.The expression of TGF-β,VEGF,Ⅰ,Ⅲ,Ⅳ and Ⅴ collagen in the lungs were down-regulated in different groups,and the combined effect of the first group was better.Experiment 3:Effect of Optimized Formulas of Effective Components of Astragalus Angelica on Newly Associated Genes of Lung Cells.On the basis of experiment one and two,the optimal group was selected as the optimized group 1,and the lung epithelium preparation,BASCS staining and Sca-1,EPCAM and AQP5 were performed in the lung tissue along with the normal group and the model group,SPC-related genes were detected.The results showed that the gene content Q2 of the model group decreased compared with the normal group.Compared with the model group,the gene content Q2 of the optimized group 1 was significantly increased,which might be related to the effective component of astragalus angelica Lung cell newborn.Conclusion:The optimal treatment group 1 is the best treatment group,that is,the proportion of the extract of Astragalus water extract and Angelica sinensis is larger,and the ratio is close to 1:1,the best effect on the prevention and treatment of pulmonary fibrosis,the other proportion optimization group Is not the best choice for the program.And inhibit the expression of TGF-β gene,VEGF,type Ⅰ,Ⅲ,Ⅳ and Ⅴ collagen genes in the lungs and promote the expression of Sca-1,EPCAM,AQP5,SPC,but its specific mechanism remains to be further studied. |
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