Anti-invasion And Migration Mechanism Of 2,3-indole Quinone In Human Neuroblastoma SH-SY5Y Cells

Background: Isatin is widely present endogenously in both human and other mammalian tissues and fluids likely as a result of the tryptophan metabolic pathway. Isatin showed significant inhibiting effect on the growth of SH-SY5 Y neuroblastoma cells. The objective of this study was to investigate that isatin is also able to alter the invasion and migration ability of SH-SY5 Y cells and probably mechanism. Methods: SH-SY5 Y cells were exposed to isatin at various concentrations(100, 200 and 400 μmol/L). Invasion assays were conducted to detect the invasiveness and scratch assay was used to analye metastasis. MMP2 and MMP9 m RNA was analyzed by Real Time RT-PCR. MMP2, MMP9 and p STAT3 protein were analyzed by Western blotting. Results: Isatin caused the most significant inhibition of cell migration and invasion. The inhibition rates of invaded cells were 42.14 % and 87.42 %, respectively(P<0.01). Wound-healing assay showed that cell migration was reduced in 200 μmol/L group compared with the control group at 36 h and 48 h, respectively. The inhibition rates of migrated cells were 42.78 % and 45.03 %, respectively(P<0.05). Treated with isatin, the expression levels of MMP2 and MMP9 m RNA and protein was decreased(P<0.05). Moreover, phosphorylated STAT3 was decreased in SH-SY5 Y cells with isatin in a concentration-dependent manner(P<0.01). Conclusions: Isatin can inhibit migration and invasiveness probably by reducing MMP2 and MMP9. The reason leading to these alterations might be that isatin down-regulates the level of p STAT3.