| Objective: This study mainly focused on IL-6, IL-18, Tumor Necrosis Factor alpha(Tumor Necrosis Factor alpha, TNF alpha) gene polymorphism and Qingdao region, such as recurrent spontaneous abortion(recurrent what abortion, RSA) risk of relevance, from gene level to discuss the reason of disease of RSA.Methods: Choose between January 2014 and June 2015 in Qingdao Women and Children hospital of 60 patients with recurrent abortion as cases, At least a normal pregnancy women in early pregnancy 60 cases as control group, Peripheral blood and extract DNA,The IL-6 rs1800795, rs1800796;IL-18 rs187238;TNF alpha rs361525, sequencing rs1799964 polymorphic loci, Use of statistical software to analyze the sequencing results, this paper discusses five loci gene polymorphism and the relationship between the risk of recurrent spontaneous abortion.Result:(1) IL-6 rs1800795 recurrent abortion group(G/C) loci GG, GC +CC genotype frequency distribution is no statistical difference(P>0.05);Rs1800796(C/G) of CC, CG and GG genotype frequency distribution in the case group and the control group were 60.00%, 40.00%, 0.00% and 33.3%, 50.00%, 16.67%, and the distribution frequency statistical differences between the two groups(P<0.001);Allele frequency of the relative risk analysis found that the relative analysis of the G allele frequency OR = 0.350, 95% CI(0.197-0.623);(2)IL-18 rs187238(G/C) of GG, GC + CC distribution frequency in the case group and the control group was statistically significant(P< 0.002), OR = 4.500, 95% CI 1.656-12.229;(3)TNF alpha rs361525(G/A) of GG, GA + AA distribution frequency in the case group and the control group was statistically significant(P < 0.002), the OR value and 95% CI value is 4.5(1.656 12.229); Rs1799964(TT, T/C) TC + CC distribution frequency in the case group and the control group was statistically significant(P< 0.003), OR = 3.000, 95% CI 1.424-6.319.Conclusion: The results of this study showed that,(1) IL-6 rs1800795(G/C) no obvious correlation with RSA risk; Rs1800796(C/G) : G allele may reduce the risk of RSA, and allele C may increase the risk of RSA;(2)IL-18 rs187238(G/C): GC genotypes and alleles C may increase the risk of RSA;(3)TNF alpha rs361525(G/A) site GA genotype might be the risk factor of RSA, allele G may reduce the risk of RSA.Rs1799964(T/C) loci alleles C may increase the risk of RSA.Objective: To explore gene expression and to bend progesterone spuc medicine treatment effect, to provide more specific basis for future treatment.Methods: Cases patients blood samples ready, Follow-up to bend progesterone drugs spuc treatment effect. According to the result of gene sequencing, there will be A significant IL-6 rs1800796(C/G), IL-18 rs187238(G/C), TNF alpha rs361525(G/A) and rs1799964(T/C) loci genotypes and alleles and spuc results compare analysis, explore the correlation between the two.Results:(1)IL-6 rs1800796 CC(C/G) loci, CG genotypes in spuc distribution frequency of success and failure spuc group respectively 57.89%, 42.11%, 57.89%, 42.11%, and no statistical difference between two groups(P > 0.05).(2)IL-18 rs187238 GG(G/C) loci, the GC genotypes in spuc distribution frequency of success and failure spuc group respectively 76.32%, 23.68% and 72.73%, 27.27%, no statistical difference between two groups(P>0.05).(3)TNF alpha: GG rs361525(G/A) loci, the GA genotype spuc distribution frequency of success and failure spuc group was 76.32%, 23.68% and 50.0%, 50.0%, statistical differences between two groups(P < 0.05),;Rs1799964 TT genotype(T/C) loci, the TC genotype spuc distribution frequency of success and failure spuc group are 23.68%, 76.32% and 50.0%, 50.0%, there is difference between two groups(P<0.05).Conclusion: IL-6 rs1800796(C/G) and IL-18 rs187238(G/C) genotypes and flexor progesterone drugs such as spuc effect had no obvious relevance;TNF alpha rs361525(G/A) in the GG genotype and rs1799964(T/C) TC genotype in RSA patients to bend progesterone spuc slightly higher success rate.Objective: IL-6, IL-18, TNF alpha gene polymorphism and recurrent miscarriage association study of existing data summary assessment.Methods: Collected through document retrieval database both at home and abroad, has been published about the IL-6, IL-18, TNF alpha gene polymorphism and recurrent abortion correlation case-control study literature. According to the inclusion criteria, determine the conform to the requirements of the documents, Rev Man5.2 application software, to include data analysis, Based on the heterogeneity of the study, a random effects model merger or fixed effects model analysis;Combining funnel figure identification of publication bias.Results: This Meta analysis, a research paper in 17(including this study), a total of 2739 cases of RSA patients and 2612 cases of normal pregnancy pregnant women.IL-6 rs1800795 involved(G/C) loci with 4 references, case group 497 cases, control group 577 cases.Involving the IL-6 rs1800796(C/G) loci with 5 references, case group 403 cases, control group 404 cases.Involves IL-18 rs187238(G/C) loci with 5 references, case group 588 cases, control group 545 cases.TNF-alpha rs361525(G/A) loci with 7 references, case group 1431 cases, control group 1266 cases.No publication bias in the literature.Meta analysis showed that IL-6 rs1800795(G/C) no obvious correlation with RSA, merger OR a value of 1.09, 95% CI(0.61-1.95);IL-6 rs1800796(C/G) merger OR value and 95%CI 0.54(0.40-0.75), Z=3.76, P<0.05, suggesting that this locus mutation are protective factors of RSA.IL-18 rs187238(G/C) site, a merger OR value and 95% CI 3.05(1.15 8.11), showed that the GC genotypes with RSA related disease.TNF-alpha rs361525 loci(G/A), merger OR value and 95% CI 1.69(1.35-2.11), that are associated with the onset of RSA.Conclusion:(1) IL-6 rs1800795(G/C) polymorphism and the risk of the disease by RSA is no significant relationship;Rs1800796(C/G):G allele can reduce the risk of RSA, allele C can increase the prevalence of RSA.(2)IL-18 rs187238(G/C) polymorphism may be associated with the onset of RSA in China.(3)TNF-alpha rs361525(G/A) loci alleles of A gene may increase the risk of RSA. |
PDF Full Text Request