Expression Of AQP-1, AQP-4, VEGF, SE-selectin In Lung Hypoxia Metabolism

Objective:To study the expression of the rats AQP-1、AQP-4in pulmonary hypoxia metabolism,further investigate the relation between AQP-1、 AQP-4and pulmonary hypoxia metabolism.Method:30SD rats were randomly divided into control and experimental groups of A,B,C and D(1/2h one-lung hypoxia group、1h one-lung hypoxia group、2h one-lung hypoxia group.4h one-lung hypoxia group). The control group performs tracheotomy tubes and two-lung ventilation. The experimental groups perform tracheotomy tubes and one-lung ventilation. Establish acute hypoxic pulmonary injury model. Take lung issue of non-ventilation after the experimental groups performed one-lung ventilation for1/2h、1h、2h、4h respectively. Their morphology was observed using optical microscopy method. The distribution of AQP-1,AQP-4on the cell membrane was observed with immunohistochemistry, and the expression of AQP-1, AQP-4mRNA was detected with real-time PCR.Result:It was observed that there was hyperaemia in experimental groups. Meanwhile, alveolar septum broaden and inflammatory cell infiltration were also observed with time.No pathological changes were observed in the control group. With immunohistochemistry the expression of AQP-1were decreased significantly with time, while AQP-4did not change. The expression of AQP-1mRNA in4h one-lung hypoxia group was significantly decreased than in the control group (P<0.05) and decended with prolongation of time (A>B>C>D), but The expression of AQP-4mRNA were no significant changed.Conclusion:The ultrastructure change of rat lung tissue can be induced by pulmonary hypoxia metabolism, which decreased expression of AQP-1, and it showed a negative correlation with it. And AQP-4showed no relation with pulmonary hypoxia metabolism. Objective:To study the expression of the rats VEGF、sE-Selectin in pulmonary hypoxia metabolism,further investigate the relation between VEGF-. sE-Selectin and pulmonary hypoxia metabolism.Method:30SD rats were randomly divided into control and experimental groups of A,B,C and D (1/2h one-lung hypoxia group、1h one-lung hypoxia group、2h one-lung hypoxia group、4h one-lung hypoxia group). The control group performs tracheotomy tubes and two-lung ventilation. The experimental groups perform tracheotomy tubes and one-lung ventilation. Establish acute hypoxic pulmonary injury model. Take lung issue of non-ventilation and blood serum after the experimental groups performed one-lung ventilation for1/2h、1h、2h、4h respectively. The level of sE-Selectin in blood serum and VEGF in blood serum and lung tissue were determined by sandwich enzyme-linked immunosorbent assay(ELISA).Their morphology was observed using optical microscopy method.The distribution of VEGF on the cell membrane was observed with immunohistochemistry, and the expression of VEGF mRNA was detected with real-time PCR.Result:It was observed that there was hyperaemia in experimental groups.Meanwhile, alveolar septum broaden and inflammatory cell infiltration were also observed with time.No pathological changes were observed in the control group. Compared to control group, the levels of sE-Selectin in blood serum were increased significantly after one lung ventilation for1h,2h,4h (P <0.05), and the levels increased with time. The levels of VEGF in lung tissue were decreased significantly (P<0.05), while it in blood serum increased significantly after one lung ventilation for4h (P<0.05). With immunohistochemistry the expression of VEGF were decreased significantly with time. The expression of VEGF mRNA in4h one-lung hypoxia group was significantly decreased than in the control group (P<0.05) and decended with prolongation of time (A>B>C>D).Conclusion:The ultrastructure change of rat lung tissue can be induced by pulmonary hypoxia metabolism, which increased expression of sE-Selectin, and it showed a positive correlation with them. While VEGF in rat lung tissue showed a positive correlation with pulmonary hypoxia metabolism time.