Detection Of Ions And Small Molecules Based On Nanoparticle Dynamic Light Scattering Technology

Nanometerials have special physical and chemical properties,and they have properties that ordinary conventional materials do not possess.After the introduction of nanotechnology in the field of metal ion detection and biological small molecule detection,the efficiency and sensitivity of the detection are improved.The high stability of colloidal nano materials based on excellent photostability,low toxicity and excellent properties such as small particle size,and the advantages of excellent fluorescent luminescence effect,ultra sensitive detection technology combined with dynamic light scattering,small biological molecules to establish a good performance and a metal ion detection and fluorescence quenching by new way the new material.This thesis is devoted to the exploration of the detection of small biological molecules and metal ions in nanomaterials.The main research work is as follows:?1?Based on sulphur modified silver nanoparticles,the use of synthesis of silver nanoparticles with lead ions formed between T-Pb²⁺-T combination principle,lead to the reunion of silver nanoparticles and its hydraulic diameter changes,the use of DLS dynamic light scattering detection method,based on the analysis of different concentrations of lead ion added after silver nanoparticles size change data,realizes the quantitative detection of lead ion.Through colorimetric method comparative study on the detection and fluorescence detection means under the condition of different ph of solution,dynamic light scattering detection system can detect lower concentrations of sensitive lead ions.This technique is very simple,sensitive and efficient.?2?Based on dynamic light scattering method and the combination of the silver nanoparticles,using silver nanoparticles and the strong interaction of dopamine causes the reunion of silver nanoparticles,and causes the hydraulic diameter of silver nanoparticles produce the principle of change,through the analysis of the dynamic light scattering method to detect different concentrations of dopamine changes as a result of the silver nanoparticles size,so as to realize the quantitative detection of dopamine.Further compared with colorimetric method detection means under the condition of the system for alkali solution is studied,the dynamic light scattering detection system still can track to the advantage of different concentrations of dopamine.In addition,silver nanoparticles and the interaction of the dopamine in about 20 minutes,the process can be directly by dynamic light scattering method for real-time monitoring of the change of particle to track,it also makes the dynamic light scattering detection means can become a potential tool for dopamine detection provides real-time detection.?3?Based on threonine and 11-mercapto naphthalene acid modified functional fluorescent nano gold material,at room temperature,using threonine as a reducing agent and 11-mercapto naphthenate as sealants,successful synthesis of a simple orange red double ligand functional nano gold clusters,this method has excellent synthesis of gold nanoparticles cluster near infrared,good colloidal stability and dispersion;In the same conditions,the use of gold nanoparticles cluster whether the fluorescence quenching of several metal ions detection,found that when mercury ions from 37.5 to 3750 nM has good linear relationship,the method is simple and quick,the advantages of the test results visualization,used for detection of mercury ion in aqueous solution.