Study On Tissue Culture Of Hair Acid Pulp And Tongken Acid Pulp

Physalis pubescens L.and Physalis tungkenensis are not only endowed with high edible and ornamental values,but also progressively regarded as important medicinal plants,thus bearing the great significance of investigation and deep research.Basically,Physalis pubescens L.and Physalis tungkenensis were used as materials,the effects of diverse explants types,different concentration and kinds of plant growth regulators on the induction of callus,as well as on the differentiation and rooting of adventitious bud were studied.The best medium were unveiled for different phases,consequently established the in vitro culture and regeneration system for Physalis pubescens L.and Physalis tungkenensis.Theory and technical basis are provided for germplasm resources of protection,its transformation of gene and production of secondary metabolites.The main results are shown as following: 1.To establish in vitro culture system for Physalis pubescens L..For the suitable disinfection methods on Physalis pubescens L.seeds,The optimum disinfecting duration was 75% Ethanol for 10 s,followed by the 0.1% mercuric chloride treatment for 7min.The optimum disinfecting duration for the young leaves and stems was about 6min.to ensure the highest survival rate,at 93.4% of leaves and 97.6% of stems.During induction callus and culture of Physalis pubescens L.The best explants were cotyledon and hypocotyl.The optimum medium of callus is L9,which comprised of 3.0mg/L 6-BA and 0.3mg/L IAA,the highest inductivity at 97.47% and 98.12%.The optimum differentiation medium is L5,which comprised MS,2.0 mg/L 6-BA and 0.2mg/L IAA,yielding the highest inductivity at 64.75% from cotyledon and 60.10% from hypocotyl.Cotyledon differentiation rate > hypocotyl.The best rooting medium was MS + NAA0.1mg/L or MS + IBA0.1mg/L,rooting ratio was 100%.2.To establish in vitro culture system for Physalis tungkenensis.At the same treatment,rate of callus was without distinction.There was greater impact on adventitious bud differentiation rate from different explants.The optimum medium of cotyledon,hypocotyl and leaves was L5.That was MS+ 6-BA 2.0 mg/L + IAA 0.2mg/L showing the maximum inductivity at 66.78%,60.11% and 75.32%,the best explants was leaves.The best rooting medium was MS + NAA0.1mg/L,rooting ratio was 100%.3.The cotyledon and hypocotyl of either Physalis pubescens L.or Physalis tungkenensis showed a polar regeneration,in another word,cotyledon petiole exhibits a higher bud inducing ability than other parts of hypocotyl;the party proximal to the end of cotyledons is easily to be inducted into bud,while another side is more likely to be callus upon the inductions.