The Quality Evaluation Of Polygala Tenuifolia Willd. Based On Spectral-effect Relationship

Objective To establish reasonable method of evaluating quality of P.tenuifolia based on fingerprint chromatography with antioxidant and expectorant pharmacological effects.Methods(1)The P.tenuifolia from four different main producing areas,Shanxi,Hebei and so on,were extracted by 70% methanol.UPLC-DAD method was used to establish 41 P.tenuifolia fingerprints with different origins.Based on P.tenuifolia’s UPLC fingerprint chromatography,“Chinese medicine chromatographic fingerprint similarity evaluation system software” was used to establish the similarity between different origins.The common peaks’ chemical components were identified by UPLC-Q-TOF-MS.(2)UPLC fingerprints of P.tenuifolia from25 different regions were established.Antioxidant efficacy peaks were obtained by correlation analysis of common peaks with DPPH and FRAP.(3)UPLC fingerprints of P.tenuifolia from 15 different regions were established.Expectorant efficacy peaks were obtained by correlation analysis of common peaks with the expectorant experiment.(4)The quality evaluation of P.tenuifolia was based on the pharmacological effects of antioxidation and expectorant activities.Cluster Analysis was used to classify P.tenuifolia in 41 places based on antioxidant and expectorant effects.(5)The quantitative analysis of multi-components by a single marker(QAMS)was conducted with 3,6-disinapoyl sucrose as an internal reference substance.The cosine value confirmed theconsistency of QAMS and ESM methods.Results1.The fingerprint similarity evaluation software divides the P.tenuifolia in different regions into two categories.The similarity of one kind of origins was more than 0.9,and the similarity of the other origins was more than 0.8.35 common peaks were obtained for the fingerprint.The chemical composition of 29 common peaks was identified by UPLC-Q-TOF-MS.2.Correlation analysis showed 11 pharmacodynamic peaks were correlated to DPPH anti-oxidative clearance rate,1(sibiricose A5),2(sibiricose A6),3,6(polygalaxanthone III),7(polygalaxanthone Ⅷ/polygalaxanthone Ⅺ),10(DISS),11(arillanin A),13(tenuifoliside F),14(tenuifoliside C),21(tenuifoliose D),32(onjisaponin Ng).Correlation analysis showed that 10 pharmacodynamic peaks were correlated to the total antioxidant activity,1(sibiricose A5),2(sibiricose A6),3,6(polygalaxanthone III),7(polygalaxanthoneⅧ/polygalaxanthone Ⅺ),10(DISS),13(tenuifoliside F),14(tenuifoliside C),21(tenuifoliose D),32(onjisaponin Ng).3.Correlation analysis showed that there were 7 pharmacodynamic peaks correlated to biological effect,5(glomeratose A),18(tenuifoliose J),22(tenuifoliose H),32(onjisaponin Ng),33(onjisaponin J),34(onjisaponin T),35(onjisaponin W/onjisaponin Fg).4.The quality evaluation were conducted with the biological effects of antioxidant and expectorant in P.tenuifolia from 41 different sources.According to the DPPH anti-oxidative clearance rate in different places,the 41 producing areas were divided into three clusters: IC50 value of P.tenuifolia from Shanxi Daning,Henan Puyang,Shanxi Yonghe was between 0.72 and 0.752 mg.The cluster II’s DPPH anti-oxidative clearance rate was the best.Shanxi WenxiDongzhen,Shanxi Xinjiang Beizhang were clustered into I category.The IC50 of these P.tenuifolia was all above 1.828 mg.The DPPH anti-oxidative clearance rate was the worst;The rest of the origins were in cluster III.The DPPH anti-oxidative clearance rate was between 0.992 and 1.565 mg,and this antioxidant capacity was better.The cluster analysis was performed based on the total antioxidant capacity of different origins.Shanxi Fenxi Heping,Shanxi Yonghe,Shanxi Da’ning and Henan Fuyang were clustered into I category.The total antioxidant value of these places was above 0.080 μmol,and the total antioxidant capacity was the best.Cluster II had 11 origins,the total antioxidant value in 0.068 ~ 0.077 μmol.The total antioxidant value of cluster II was better.There were 26 origins of P.tenuifolia and their total antioxidant value was in0.052 ~ 0.064 μmol.And this places total antioxidant capacity was the worst.The41 producing areas were divided into three categories based on the expectorant biological value.Shanxi Xin Jiang Hengqiao,Shanxi Jishan Taiyin,Shanxi Wenxi Peishe,Shanxi Jishan Daiyu,Shanxi Wenxi Xuedian,and Shanxi Wenxi Dongzhen were clustered into I category.In this category,the expectorant biological value was higher than 151.15 U/g,and expectorant biological value was the best,Cluster II has 21 places.The expectorant biological value was in 89.713~134.13 U/g.The expectorant capacity was better.There were 14 habitats of P.tenuifolia in cluster III,The expectorant biological value of these places was between 47.185 and77.443 U/g,and the expectorant ability was the weakest.5.QAMS and ESM methods showed that the cosine similarity of the two methods was more than 0.999920,which indicated that QAMS method was established.According to QAMS and ESM methods,the maximum and minimum total concentrations of the 9 standards from 23 productions were 21.291 and12.053 mg/g,respectively.It showed differences between different P.tenuifoliasamples.ConclusionIn this study,UPLC-Q-TOF-MS was used to identify the chemical constituents of the common peak of P.tenuifolia combining with its pharmacodynamics.The pharmacodynamic peaks were obtained through the correlation analysis of expectorant and anti-oxidation pharmacodynamics.To evaluate the quality evaluation model of P.tenuifolia and improved the insufficiency of a single index to assess the quality evaluation.It ccould provide ideas for quality evaluation of traditional chinese medicine.