Model Simulation And Circuit Design Based On Nanosecond Pulses Working On Cells

At present,the incidence of cancer in the world ranks second,making it one of the major killers that are second only to cardiovascular disease and threaten human health.According to the WHO survey,there are more than 40 million cancer patients worldwide.With the rapid industrial and urbanization process and increasing environmental pollution,the incidence of cancer is still increasing at a rate of 2-3% per year.Therefore,it is very urgent and important to find an effective means to treat cancer.Current methods for treating tumors include surgery,chemotherapy,radiotherapy,immunotherapy,and their combined use.For some patients,these programs increase their survival rate by five years or longer.However,these programs have many important limitations: such as tumor recurrence and metastasis,the formation of drug resistance,toxic side effects,reduce the patient’s immunity,can’t remove important organ tumors,causing damage to the appearance,hair loss caused by radiation,these side effects often make the treatment fail.Many scholars try to develop a treatment plan that can avoid the above side effects.Therefore,in recent years,studies on the influence of pulsed electric fields on tumor cells and biological tissues is of interest to scholars,especially the bioelectric effect of nanosecond pulsed electric field.The effect is because it has a specific effect of inducing apoptosis of tumor cells.The pulsed electric field with tumor targeting and apoptotic minimally invasive treatment can induce tumor apoptosis during clinical tumor treatment,and the damage to normal tissue around the tumor can be greatly reduced.Therefore,the study of cell electroporation caused by nanosecond pulse can not only promote the clinical treatment of tumors,but also reduce the suffering of cancer patients,improve their quality of life and promote social progress,which is of great significance.In this paper,the formulas of capacitance and resistance of spherical shells and spheres are deduced,the transfer functions of various parts of cells are deduced,and the time domain differential equations are deduced.A spherical single cell all-circuit equivalent model was constructed based on a cell bilayer dielectric model.Using MATLAB,the cell membrane transmembrane potential at nanosecond pulsed electric fields was quantified by programming and Simulink.Using COMSOL,the frequency dispersion effect of each component of the cell was introduced,the transient calculation was completed,the steady-state calculation was performed,the parameters were extracted,and the membrane voltage was quantitatively calculated.When the continuous nanosecond pulse was used,the membrane voltage amplitude and the nuclear membrane voltage amplitude increased with the increase of the pulse number,indicating that the nanosecond pulsed electric field has a cumulative effect on the cell membrane and nuclear membrane.At nanosecond pulse widths,the voltage across the cell and nuclear membranes increases with increasing pulse width,indicating that the nanosecond pulsed electric field has a strong effect on cell membranes and nuclear membranes.The thinner the cell structure,the stronger the field strength,indicating that the nanosecond pulsed electric field has an effect on the cell membrane and internal membrane structures.Using Multisim,build a high-voltage pulse source using MOSFETs and verify the circuit in Simulink.Debug the circuit,make the PCB,complete the actual test,and realize the no-load output of the pulse source.The simulation results and pulse source circuits provide guidance and preparation for subsequent experiments.