Comparative Study Of Radix Hedysari And Radix Astragali On Anti-osteoporosis?Antioxidant And Hepatoprotective Activities

Radix Hedyseri and Radix Astragali belong to the fabaceae family,The former is the dry root of Hedysarum polybotrys Hand-Mazz,named for its ruddy skin,and the latter is prepared from the dry root of the Astragalus membranaceus?Fisch.?Bge.var.mongholicus?Bge.?Hsiao or Astragalus membranaceus?Fisch.?Bge.Both of them are commonly considered as one famous-region drug of Gansu province in China.Radix Hedyseri is often used as the substitute of Radix Astragali,and in some regions Radix Hedyseri is esteemed as the uppermost class of Radix Astragali.Actually these two herbs were classified under the same material medica in China Pharmacopoeia until 1985 edition.At present,there are many studies on Radix Astragali at home and abroad,but little is known about the pharmacological effects of Radix Hedyseri.The dissertation focuses on three aspects,the specific research contents are as follows:The first part:To investigate the anti-osteoporosis effect of ethanol extract from Radix Hedysari and Astragali?HE/AE?in ovariectomized?OVX?rat model.A total of 72 female Sprague-Dawley?SD?rats aged 3 months were randomly divided into nine groups with eight in each group,the rats in the sham control group,model group,0.2 mg/kg body weight in every day of estradiol valerate group as positive control,HE1^HE3 and AE1^AE3treated groups,respectively.Two weeks after surgery,each group was respectively given their treatment by gavage.On the last day of 12 weeks of continues administration,bone mineral density,the levels of serum Ca and P,and the activities of tartrate-resistant acid phosphatase?StrACP?and alkaline phosphatase?AKP?were measured.The bone mineral density and bone biomechanical properties were determined in the right thighbone.The results showed that HE/AE could enhance bone mineral density?P<0.05 or P<0.01?,improve modulus of elasticity of bone,maximum load and maximum stress?P<0.05 or P<0.01?,increase the level of serum Ca,P and AKP,and decrease StrACP?P<0.05 or P<0.01?.HE/AE have a distinct and preventive effect on osteoporosis of rats.The second part:To evaluate the antioxidant effects of aqueous fractions from Radix Hedysari and Radix Astragali?HAF/AAF?in vivo and in vitro.?Antioxidant studies in vitro:Compared with Vc,the vitro antioxidant activities were evaluated by 1,1-diphenyl-2-picrylhydrazyl?·DPPH?radical scavenging,the hydroxyl radical scavenging?·OH?,and potassium ferricyanide reduction.The results showed that HAF/AAF groups had good antioxidant activities in vitro,and HAF groups had a better effect than AAF groups.?Antioxidant study in vivo:Oxidative stress mouse models induced by D-galactose were used.Compared with VE,the vivo antioxidant activities of extracts were studied by measuring total superoxide dismutase?T-SOD?,glutathione peroxidase?GSH-PX?activity and malondialdehyde?MDA?content of serum,liver and Brain tissue.It found that HAF/AAF groups could obviously increase SOD,GSH-PX activity,and decrease the content of MDA in serum,liver and Brain tissue of mouse.HAF/AAF groups showed a significant effect compared with the model group?P<0.05 or P<0.01?.The experimental results can be confirmed that HAF/AAF groups had antioxidant activity both in vitro and in vivo,improved oxidative stress mouse models induced by D-galactose.The third part:To investigate the prevention and cure effect of aqueous fractions from Radix Hedysari and Radix Astragali?HAF/AAF?on hepatic oxidative stress in mice with liver injuryinduced by carbon tetrachloride?CCl₄?.Ninety Kunming mice were randomly divided into nine groups?ten for each?:model control group;colchicine treated group?0.6 mg/kg?;normal control group;HAF1³ and AAF1³ treated groups.All mice except those in the normal control group received a subcutaneous injection of neck and back of 20%CCl₄?Once every 5 days?while mice in the other group were received an intragastric administration of HAF/AAF or colchicine every day.After 42d,the levels of serum alanine aminotransferase?ALT?,aspartate aminotransferase?AST?,total protein?TP?,album protein?ALB?,and the ratio of the ratio of album protein/globin protein?A/G?were assayed.SOD,GSH-Px and the contents of MDA in liver were measured.The result was indicative of HAF/AAF could inhibit the increasing of ALT,AST in serum of mice?P<0.05 or P<0.01?,increase the level of the contents of ALB and the SOD,GSH-Px activities in liver tissue?P<0.05 or P<0.01?,decrease the MDA content in liver?P<0.05 or P<0.01?.These findings suggest that HAF/AAF reduces hepatic oxidative stress in mice with acute liver injury induced by CCl₄,thereby showing the protective effect on mice with acute liver injury induced by CCl₄.