Preparation Of Selenium Nanoparticles Decorated By Laminarin Polysaccharides And Its Growth Inhibition On Human Liver Carcinoma Cells

Objective:Laminarin polysaccharides(LP)are natural β-1,3-glucose polymers.LP are widely used in the field of biomedicine because of their biological activities and high security.In this study,the LP decorated selenium nanoparticles(LP-SeNPs)were mono-disperse spherical morphology with small particle size and powerful anti-hepatoma activity.Moreover,many molecular biological methods were used to investigate the potential anti-hepatoma mechanism of LP-SeNPs.Methods:LP-SeNPs were prepared by using laminarin polysaccharides as modifiers and stabilizers.Transmission electron microscope(TEM),laser particle analyzer,UV-visible spectrometer and Energy dispersive X-ray spectrometer(EDS)were used to characterize the prepared SeNPs.The anti-proliferation effects of LP-SeNPs were explored by MTT assay,cell apoptosis was detected using Annexin V-FITC/PI apoptosis detection kit.The effects of LP-SeNPs on the expression levels of Bcl-2,Bax,Caspase 9,LC3 and p62 in HepG2 cells were detected by Western blot.The lysosomal acidity and activity were evaluated to observe the effects of LP-SeNPs on lysosome.Results:In the present study,laminarin polysaccharides(LP)decorated selenium nanoparticles(LP-SeNPs)with an average diameter of 60 nm were synthesized.The results revealed that LP-SeNPs resulted in a dose-dependent cytotoxicity and apoptosis of HepG2 cells.Additionally,the nanoparticles may first localize in the lysosome,and then released into cytosol to activate the downstream signals.Moreover,LP-SeNPs significantly up-regulate the expression of Bax and cleaved Caspase 9,as well as down-regulate the level of Bcl-2,which suggested that LP-SeNPs induced mitochondria-mediated apoptosis.Furthermore,exposure of LP-SeNPs for 12 h induced the up-regulation of LC3-II and p62.Further investigation revealed that LP-SeNPs inhibited the late phase of autophagy by influencing the function of lysosome.Conclusion:LP-SeNPs exhibited significant anti-proliferation effects against human liver carcinoma cells.Further mechanism investigations indicated that LP-SeNPs exerted their cytotoxicity in HepG2 cells by inhibiting autophagy and inducing apoptosis.This study will provide experimental and theoretical basis for further research on anti-liver cancer drugs.