Enhancement Of Bone Regeneration Of Cranial Defects Through BMP2 Polypeptide Modified With 7E

With the ageing population and the popularity of oral implants,how to promote or accelerate bone regeneration and repair bone defects is increasingly important for improving the quality of Patients’ lives and maintaining a healthy mental state.It has become an hot topic among oral and maxillofacial surgery,oral planting,and orthopedic surgery.This project is in view of this issue.We have designed and prepared 7E modified BMP2 polypeptide,and carried out a series of comprehensive physical characterization,in vitro and in vivo studies to evaluate its role in the repair of skull defects.The main findings and conclusions are as follows:1.Scanning electron microscopy show that the 7E modified BMP2 peptide does not change the original appearance of bone powder.Analysis by X-ray photoelectron spectroscopy,7E modified BMP2pep can effectively promote the adsorption of polypeptide on the surface of bone powder,and the quantitation of absorbed peptide is most in the highest concentration of 7E experimental group.In vitro release experiments,from the cumulative release curve of nearly 384 hours can be seen that in the KIP group show explosive release in the first 24 hours,while the 7E group is relatively slow release,the knuckle epitopes within BMP2pep could adsorb on the bone powder.On the other hand,sustained and controlled release is controlled.This combination is beneficial to the stability of bone meal performance.2.In vitro experiments,ALP quantitative analysis,alizarin red staining,and PCR detection from molecular levels were employed to evaluate the effect of BMP2pep on the osteogenic differentiation of BMSCs.The results further confirmed that 7E can significantly promote the efficient adsorption of BMP2pep on the surface of bone powder.It was proved that the 7E modified BMP2 can more effectively promote the osteogenic differentiation of BMSCs through osteogenic markers.3.In vivo experiments,Micro-CT and Masson’s trichrome staining demonstrated that the 7E modified BMP2pep could increase its binding rate to bone powder,which could make it perform good function in vivo and have good biocompatibility.The effect of sexual and induced osteogenesis is more pronounced.In summary,the 7E modified BMP2pep bone powder prepared in this study is a novel bone augmentation material with simple,convenient,low cost and excellent clinical application prospect.