Expression Of Toll-Like Receptor 2 In Colon Tumor And Its Effects On Cell Growth

Objective:To observe the expression of Toll-like receptor-2(TLR2)in colon tumor and its effects on cell growth by activating TLR2 signaling.Methods:Immunohistochemistry was used to detect the protein expression of TLR2 in twenty paired colon cancer patient tissue samples and normal adjacent tissue samples.Stimulated with TLR2 ligand Pam3Cys(P3C),CCK-8,wound healing and Transwell assays were used to observe effects on proliferation,migration and invasion of colon tumor cell line SW480 and HCT116.Western Blot was used to measure protein expression of PI3K/Akt and NF-κB signaling pathways with P3C treatments.Results:Positive rate of TLR2 expression in colon tumor tissues is 85.0%(17/20),while that in adjacent normal tissues is 40.0%(8/20),and the expression of TLR2 in the colon tumors was shown to be significantly high compared with adjacent normal tissue using immunohistochemistry(χ~2=8.64,P<0.01).Proliferation,migration and invasion of TLR2-driven colon tumor cells were elevated(P<0.05).PI3K/Akt and NF-κB signaling pathways were up-regulated in TLR2-driven colon tumor cells(P<0.05).Conclusion:TLR2 is highly expressed in colon tumor,and promotes proliferation,migration and invasion of colon tumor cells via PI3K/Akt and NF-κB signaling pathways.