The Inhibitory Effect Of Rosiglotazone On The Retinal Inflammatory Response In Diabetic Rats

ObjectiveTo investigate the protective effect of rosiglitazone on retina Müller cells in diabetic mellitus(DM)rats.MethodsOur study used male SD rats with intraperitoneal injection of streptozocin(STZ)to establish DM models.Then we gave the models gavage of rosiglitazone(RSG)solution for inhibiting the inflammatory response to establish the DM-RSG group.With gavage of clean nutrient feed,we established the DM group.And with gavage of the equal volume of normal saline to the normal SD rats,we established the countrol group.After 12 weeks,we detected the rats’ quality,blood glucose concentration and serum S100A8/A9 protein concentration.We used immunofluorescence and optical density to analyze glial fibrillary acidic protein(GFAP)which is the activation markers of müller cells and western blot to measure the change of intercelluler adhesion molecule(ICAM-1),tumor necrosis factor(TNF-α)and GFAP expression in semiquantitative by Western blot.ResultsCompared with the control group,the quality of rats in the DM and DM-RSG group is significantly reduced(P < 0.01),and blood glucose is elevated(P < 0.01).Compared with the DM group,the quality of rats in the DM-RSG group is not significantly reduced(P > 0.01),but the blood glucose is reduced(P < 0.01).The overall comparison of serum S100A8/A9 concentrations in the three groups is statistically significant(F=117.62,P < 0.01).Compared with the control group,the concentration of S100A8/A9 in the DM group increases(P < 0.01)and compared with the DM group,the concentration in the DM-RSG group decreases significantly(P < 0.01).Immunofluorescence shows that GFAP is expressed in the inner boundary layer,nerve fiber layer and the retinal ganglion cell layer in the control group,but besides the inner boundary layer and the retinal ganglion cell layer,it is expressed in both the inner and outer plexiform layer in the DM group and DM-RSG group.And the expression of GFAP measured by optical density is higher in DM group and lower in DM-RSG group compared with the control group.The expression of GFAP,ICAM-1 and TNF-α measured by Western blot is higher in DM group than in the control group(P < 0.01)and lower in DM-RSG group than in DM group(P < 0.01).ConclusionOur study suggested the resiglitazone can reduce diabetic retinal inflammation,protect Müller cell and may have a potential therapeutic effect on diabetic retinopathy.