| ObjectiveIn this study,tandem mass spectrometry metabolomics detection technique was used to detect and analyze dry blood spot(DBS)samples of patients with effective colon cancer and liver metastases and those with no metastasis of colon cancer.The value of amino acids in the diagnosis and efficacy evaluation of colon cancer with liver metastasis.MethodsNinety-five cases of therapeutically effective colon cancer patients with hepatic metastases before and after every two cycles of dry blood spot(DBS)samples were analyzed for amino acid metabolism using tandem mass spectrometry metabolomics.At the same time,DBS samples from 87 patients with colon cancer without metastasis were also tested.To compare the serum amino acid metabolism levels of patients that have colon cancer with liver metastasis and colon cancer without metastasis.To find out the amino acids that are different between them.Then compare the before and after treatment of these amino acids with difference in the patients that have colon cancer with liver metastases to find the amino acids with significant differences.We also compare the significant amino acid levels before and after treatment with different therapeutic effects,and analyze the relationship between their levels and efficacy,and further identify metabolites with the potential for diagnosis and therapeutic potential of colon cancer with liver metastases.ResultsBy comparing the serum amino acid level of colorectal cancer with liver metastasis patients and colon cancer without metastasis patients,it was found that 7 amino acid levels were significantly different.Among them,the concentrations of aspartic acid,asparagine,glutamic acid and tryptophan had statistical significance(P <0.05).The three amino acid concentrations of lysine,phenylalanine and tyrosine were significantly different(P<0.01).Compared with pre-treatment,there were some changes in 7 different amino acid levels after treatment for patients with colon cancer and liver metastases.However,only the concentration of tryptophan changed significantly in all three comparisons after treatment.Lower serum tryptophan levels in patients with colon cancer and liver metastases.After systemic chemotherapy,its concentration increased significantly compared with that before treatment(P<0.05).After comparing the serum tryptophan levels in patients with different therapeutic effects,it was found that the amino acid levels differed among patients with different curative effects and the difference was statistically significant(P<0.05).ConclusionsTThis study shows that DBS metabolomics detection by direct injection of tandem mass spectrometry can be an effective method for predicting hepatic metastasis in colon cancer patients.The serum concentrations of seven amino acids represented by tryptophan,including aspartic acid,asparagine,glutamic acid,lysine and so on,change during liver metastasis of colon cancer,among which tryptophan may become colon cancer with liver metastasis.For patients with colon cancer and liver metastases,tryptophan may be a potential biological marker with diagnostic and therapeutic value. |
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