Study Of TCM Clinical Syndrome Type Of Esophageal Carcinoma And The Mechanism Of The Inhibitory Effect Of Tongge Decoction On The Proliferation Of Esophageal Cancer Cells

Background:Esophageal cancer is a common malignant tumor of the digestive system.It often has no obvious symptoms at the early stage,and most of them are malignant tumors in the middle and late stages.The incidence and mortality of esophageal cancer in China are the first in the world,including Feicheng in Shandong,Cixian in Hebei and Yangzhong in Jiangsu as high incidence areas.Surgical treatment is often difficult to cure or relapse after surgery.Currently,the treatment of esophageal cancer mostly adopts comprehensive therapy.The role of traditional Chinese medicine in the comprehensive treatment of esophageal cancer has attracted people’s more and more attention.The standard of TCM syndrome type is the key to improve the curative effect of Chinese medicine for the treatment of esophageal cancer.However,there is no uniform standard for TCM syndrome of esophageal cancer at present.The scientific and clinical practicability of the standard of TCM syndrome type of esophageal cancer is still to be verified,the correlation between TCM syndrome type of esophageal cancer and modern molecular biological characteristics is still lacking in deep understanding,some data on TCM syndrome of esophageal cancer are scattered in various reports and lack of systematic summary.Therefore,it is of great significance to study the TCM syndrome type of esophageal cancer for basic and clinical research.Objective:Through clinical and experimental research,we summarized the classification and core pathogenesis of TCM Syndromes of esophageal cancer,and played the role of syndrome type in basic and clinical research.Methods:Collected from January 2016 to December 2017,Oncology Department of integrated traditional Chinese and Western medicine Hospital in Jiangsu Province,Department of oncology in Jiangsu Province Traditional Chinese Medicine Hospital,Oncology Department of Zhenjiang city of integrated traditional Chinese and Western Medicine Hospital,Oncology Department of Baoying County Hospital of traditional Chinese Medicine and diagnosed by image and pathological diagnosis as esophageal cancer.According to clinical data and related literature,self designed clinical observation form of TCM syndrome of esophageal cancer,a real record of the patient’s personal basic information,pathology,surgical methods,radiotherapy and chemotherapy,stool,tongue coating,pulse image etc,reference to the standard of TCM syndrome differentiation of cancer of the esophagus in the book "Guide to diagnosis and treatment of TCM for tumor" of the Chinese society of ChineseMedicine,conscientiously and accurately sum up the TCM syndrome type of each patient,statistical analysis by using statistical software SPSS21.0,the main TCM syndrome type and core pathogenesis were obtained,and according to Professor jiege Huo’s Empirical prescription,we conduct corresponding experimental research.Results:Clinical research part:1.The main syndrome type of esophageal cancer is intermingled phlegm and qi syndrome,blood stasis and phlegm stagnation syndrome,the elderly tend to 61-80 years of age;2.Esophageal cancerwas more occurred in the middle of the esophagus,the lower middle section,with syndrome of intermingled phlegm and qi,blood stasis and phlegm stagnation syndrome-based;3.Esophageal cancer TCM syndrome of intermingled phlegm and qi,rare pink tongue,thin white fur;Esophageal cancer blood stasis and phlegm stagnation syndrome,mostly dark purple tongue,dark red tongue,yellow moss or yellow and greasy fur.Basic experiment part:10%and 20%of the drug-containing serum had a significant inhibitory effect on the proliferation of Eca-109 cells,which the effect is enhanced with time.The percentage of G1 phase in 20%low and medium dose drug-containing serum group was significantly higher than that in control group,and the percentage of G2+S phase was significantly lower than that of control group.Compared with the control group,the apoptosis rate of the 20%drug-containing serum in each dose group was significantly increased.In addition,20%of the drug-containing serum could significantly reduce the adhesion and invasion of Eca-109 cells.The results of the experimental study further demonstrated that the core pathogenesis of esophageal cancer is intermingled phlegm and qi,blood stasis and phlegm stagnation.Provide experimental basis for the clinical application of every other soup for the treatment of esophageal cancer with the characteristics of intermingled phlegm and qi,blood stasis and phlegm stagnation.Conclusions:1.The core pathogenesis of esophageal cancer is intermingled phlegm and qi,blood stasis and phlegm stagnation;The main syndrome type of esophageal cancer is intermingled phlegm and qi syndrome,blood stasis and phlegm stagnation syndrome2.Tongge decoction containing drug serum can inhibit the proliferation of esophageal cancer Eca 109 cells,induce apoptosis and decrease the cell adhesion and invasion.