The Effects Of AKR1B10 In Human Glioma

Objective:To investigate the expression of AKR1B10 in human glioma and explore the effects of AKR1B10 in the proliferation of glioma.Materials and methods: 70 cases from January 2012 to August 2016 with different pathological grading(I-IV)of pathological diagnosis and clinical data were collected in the first people’s hospital of Chenzhou.There were 3 cases of grade I,23 cases of grade II,21 cases of grade III and 23 cases of grade IV.And 17 cases of normal tissue with pathological diagnosis were obtained.The expression levels of AKR1B10 in human glioma and normal tissues were measured by immunohistochemistry.The glioma cell lines were divided into two groups: U251/AKR1B10 group(transfected with AKR1B10)and U251/vector group(transfected with vector).The effects in growth and proliferation of AKR1B10 in two groups(U251/AKR1B10 group and U251/vector group)were detected through CCK8 and cloning formation assay.SPSS 18.0 statistical software was used to analyze the data.Results: 1.AKR1B10 has different expression level in different grading glioma and are detected mainly in the cytoplasm,and barely expressed in normal tissues.2.The expression levels of AKR1B10 in glioma are related with tumor size.3.The glioma cells are divided into twogroups: U251/AKR1B10 group(transfected with AKR1B10)and U251/vector group(transfected with Vector),then the optical densityand colony formation efficiencyare measured.Conclusion: 1.AKR1B10 is detected mainly in the cytoplasm of human glioma and barely expressed in normal tissues.The expression levels of AKR1B10 in glioma are related with tumor size(p<0.01,r=0.391),but not related with gender,age,tumor location and grade.2.AKR1B10 stimulates proliferation of glioma cells.