| Objective:To investigate the effect of cisplatin and taxol on proliferation and invasion of prostate cancer cell line PC-3 cells when combined with selective cyclooxygenase 2(COX-2)inhibitor celecoxib,potentiating clinical research of COX-2 inhibitor in cancer therapies.Materials and methods:1.The IC50 of cisplatin and taxol on prostate cancer cell line PC3 proliferation was determined respectively by MTT(4,5 – dimethyl – 2-thiazolyl)-2,5 – diphenyl – 2 – H-tetrazolium bromide)assay.Experiments were divided to the following groups: A.Group cisplatin,cells were treated with cisplatin for 24 h,the concentrations were 0,0.25,0.5,1.0,2,4 μg/ml respectively.B.Group taxol,PC-3 cells were treated with taxol for 24 h,the concentration of taxol were 0,0.25,0.5,1.0,2,4 μg/ml respectively.After treatment,cells were subjected to MTT assay.The optic density(OD value)was measured at 490 nm,and IC50 was determined.2.The effect of celecoxib combined with cisplatin or taxol on PC-3 cell proliferation was measured with MTT assay.Experiments were divided to the following groups: A.Group Asp Cis,cells were treated with 80 μM aspirin plus 0,0.25,0.5,1,2,or 4 μg/ml cisplatin.B.Group Asp + Tax,cells were treated with 80 μM aspirin plus 0,0.25,0.5,1,2,or 4 μg/ml taxol.C.Group Cel + Cis,cells were treated with 80 μM celecoxib plus 0,0.25,0.5,1,2,or 4 μg/ml cisplatin.D.Group Cel + Tax,cells were treated with 80 μM celecoxib plus 0,0.25,0.5,1,2,or 4 μg/ml taxol.Cells in these four groups were treated with indicated drugs for 24 hours,then the cell survival were measured by MTT in 490 nm absorbance.3.The effect of celecoxib combined with cisplatin or taxol on PC-3 cell invasion was measured with transwell assay.Experiments were performed as following groups: Cells were treated respectively with A.0.5 μg/ml cisplatin;B.0.5 μg/ml taxol;C.0.5 μg/ml cisplatin plus 80 μM aspirin;B.0.5 μg/ml taxol plus 80 μM aspirin;E.0.5 μg/ml cisplatin plus 80 μM celecoxib;F.0.5 μg/ml taxol plus 80 μM celecoxib.24 hours later,the trans-membrane cells were stained with crystal violet,counted in microscope with at least 5 high power fields.Results 1.A.Cisplatin inhibited PC-3 cell proliferation,with an IC50 of 0.5 μg/ml.B.Taxol inhibited PC-3 cell proliferation,with an IC50 of about 0.5 μg/ml.2.A.Aspirin combined with various concentration of cisplatin respectively inhibited PC-3 cell proliferation.The inhibition rates(%)were 0,35.67±0.02,49.84±0.08,55.17±0.09,60.31±0.12,65.43±0.11(mean ± standard deviation).B.Aspirin combined with various concentration of Taxol all inhibited PC-3 cell proliferation.The inhibition rates(%)were 0,38.79±0.07,49.12±0.08,57.65±0.21,63.78± 0.19,71.38±0.01(mean ± standard deviation).C.Celecoxib combined with various concentration of cisplatin all inhibited PC-3 cell proliferation.The inhibition rates(%)were 0,53.55±0.21,64.64±0.17,74.07±0.16,81.41±0.03,92.11±0.23(mean ± standard deviation).D.Celecoxib combined with various concentration of Taxol all inhibited PC-3 cell proliferation.The inhibition rates(%)were 0,64.59±0.39,70.26±0.12,80.96±0.01,85.68±0.01,96.88±0.15(mean ± standard deviation).3.By transwell assay,we observed that: A.cisplatin inhibited PC-3 cells invasion.The number of cells pass through membrane was 49±3.B.Taxol inhibited PC-3 cells invasion.The number of cells pass through membrane was 44±4.C.Cisplatin plus aspirin inhibited PC-3 cells invasion.The number of cells pass through membrane was 47±4.D.Taxol plus aspirin inhibited PC-3 cells invasion.The number of cells pass through membrane was 40±2.E.Cisplatin plus celecoxib inhibited PC-3 cells invasion.The number of cells pass through membrane was 26±2.F.Taxol plus aspirin inhibited PC-3 cells invasion.The number of cells pass through membrane was 23±3.sConclusion: 1.Celecoxib may enhance the inhibitory effect of cisplatin or taxol on PC-3 cells proliferation in vitro.2.Celecoxib may enhance the inhibitory effect of cisplatin or taxol on PC-3 cells invasion in vitro. |
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