Effects Of Bisphenol A On The Growth,Reproductive Development And Apoptosis In Caenorhabditis Elegans Under Different Exposure Ways

Bisphenol A(BPA),a well-known environmental endocrine disruptor,has been found to be widely distributed among the population and become a hot area in the toxicological research.For most people the primary source of BPA exposure is through the diet.Infants and children have the highest estimated daily intake of BPA in the general population.There is growing evidence suggests that exposure to endocrine-disrupting chemicals(EDCs)early in life can cause a range of disorders later in life.EDCs exopsure might adversely affect normal human development by altering the action of endogenous hormones or the endocrine system.As one characteristic of BPA is a pronounced influence on human hormonal regulation,particular focus has been given to its role in reproductive processes.With respect to reproductive effects,based on the findings from previous publications,BPA is concluded as a reproductive toxicant.The adverse effects of BPA on reproduction and development have been demonstrated in various model systems.The present study investigated the effects of BPA on the reproductive toxicity under different developmental periods and exposure ways using the model organism Caenorhabdites elegans.Our results showed that BPA did not affect the body length at the concentration of less than 10 μmol·L-1 both for solid and liquid exposure.However,the gonad development toxicity of BPA was significantly higher in liquid exposure than that in solid exposure,and the embryonic period was more susceptible compared with L1 larval stage.Moreover,though BPA could induce obvious germ cell death under solid exposure both in L1 larval and embryonic period,the latter seems to be more sensitive to BPA.Otherwise,there was no difference to be found for germ cell death between embryonic period and L1 larval stage under liquid exposure way.Through changing the exposure medium and exposure window,effects of BPA on the growth,reproductive development and apoptosis in Caenorhabditis elegans were analyzed,together the research revealed that the distinctions between biological effects were caused by different BPA exposure ways.