Apelin-13 Restores Recognition Memory Deficits And Adult Hippocampal Neurogenesis Suppressed By Chronic Stress

Object: In the present study,we evaluated that Apelin-13 plays neuroprotection effect in a variety of stress model and regulation of stress-induced study and memory impairment in rats;The antidepressant effects of Apelin at unexpected chronic mild stress model of rats need hippocampal neurogenesis to get involved.On this basis,we further studied whether Apelin-13 improves stress-induced recognition memory deficits and adult hippocampal neurogenesis suppressed in rats,then research the molecular mechanism of its action.Methods: The chronic stress rat model was established by repetitive footshock stress(RFS).The effects of Apelin-13 treatment were observed to novel object location(NOL)and novel object recognition(NOR)task in RFS-induced rats;The BrdU positive cells in the DG of hippocampus was counted by Immunocytochemistry,the effects of Apelin-13 treatment on adult hippocampal neurogenesis in RFS-induced rats were also observed;The effects of Apelin-13 treatment on phosphorylation level of GSK3β at Ser9 and the protein level of total β-catenin in hippocampus were observed by Western Blot.Results: Apelin-13 treatment reversed NOL and NOR memory taskimpairments induced by RFS;Apelin-13 restored the decrease of BrdU-positive cells of adult hippocampal neurogenesis induced by RFS;Apelin-13 reversed the increase of the level of phosphorylation of GSK3βin hippocampus induced by RFS;Apelin-13 reversed the increase of the level of total β-catenin in hippocampus induced by RFS.Conclusion: Apelin-13 reversed recognition memory deficits and adult hippocampal neurogenesis suppressed by RFS treatment,and the molecular mechanisms was associated with regulate GSK3β/β-catenin signaling.