Study On The Chemical Constituents Of Cistanches Herba And Its Neuroprotective Effect Investigation

Cistanches Herba,a traditional Chinese medicine was recently discovered to relate with significant neuroprotective effect,such as anti-Alzheimer’s disease,improving learning and memory ability,and preventing cerebral ischemia and ischemia-reperfusion,which has been a research hot spot.But few studies focus on screening and evaluating the active ingredients of Cistanches herba and elucidating the mechanism of neuroprotective effect systematically.In this study,modern chromatography separation technology was applied to separate and purificate compounds from Cistanches herba,and the structures of these compounds were elucidated according to their physical and chemical properties and spectral data.And a systems pharmacology approach that integrated active compound screening,target prediction.network construction and analysis was used to study mechanisms of neuroprotective effect of Cistanches herba.The methods and results of this study provided a guiding role for further study of the active componds of Cistanches herba,and its neuroprotective effect,which also provide new idea for study of the activity of natural active componds and development of new drugs possessing specific pharmacological activity.This study applied a variety of chromatographic separation technology isolated 6 compounds and identified the structures of them by modern spectrum technology.3 phenylethanoid glycosides:isoacteoside(compound 1),tubuloside B(compound 2)and acteoside(compound 3);2 iridoids:6-deoxycatalpol(compound 4)and 5-gluroside(compound 5);1 lignans:(+)-syringaresinol-O-β-D-glucopyranoside(compound 6)were identified by nuclear magnetic resonance spectrum.It’s worth noting that isoacteoside,tubuloside B and acteoside have been confirmed possessing significant neuroprotective effect.The comprehensive systems approach identified forty-three bioactive compounds from one hundred and thirty compounds with favorable pharmacokinetic properties.Fortunately,three phenylethanoid glycosides and one lignans were screened as bioactive compounds.which have been isolated in this study.In addition,through systematic network analyses,their targets are identified to be forty-one including twenty-five approved therapeutic targets and sixteen potential targets respectively.which are associated with thirteen neurological diseases.The results of compound-target-diseases and target-pathway networks displayed that Cistanches herba exert neuroprotective effect by regulating targets especially CTNNB1,APP,MAPT,PTGS2,CASP3,HMOXI,SOD1 and FASN as well as a set of other targets on fourteen related pathways to prevent and treat nervous system diseases.In this study,through the modern chromatographic and spectroscopic techniques six compounds were separated and identified from Cistanches herba.And this study also applied systems pharmacology method to explore the mechanisms of neuroprotective effect based on a compound-target-diseases and target-pathway networks.Ultimately,the results provided reliable theoretical basis and research foundation for further development and utilization of Cistanches herba and new ideas for the development of novel neuroprotective effect drugs.