| Objective:To study the relationship between the autophagy of human leukemia K562 cells induced by diallyl disulfide（DADS）and the PI3K/Akt signaling pathway.Methods:K562 cells were treated with 20,40 mg.L-1 DADS for 24h or K562cells were treated with 40 mg.L-1 DADS for 12,24h.After treatment,the expression levels of Akt,p-Akt,P70S6K,p-P70S6K and LC3 protein were detected by western blotting.Treated K562 cells with 40mg.L-1 DADS,Akt inhibitor（MK-2206）alone or two drug combined treated K562 cells for 24h,the expression levels of Akt,p-Akt and LC3 protein were detected by western blotting.Control groups were used as references.Results:1.The expression of p-Akt and p-P70S6K protein in K562 cells treated with 20,40 mg.L-1DADS were significantly lower than that of the control group（P<0.05）.The total protein Akt and P70S6K were no significant difference（P>0.05）.The expression level of autophagy-related protein（LC3）was higher than that of the control group（P<0.05）,and the highest in the 40mg.L-1DADS group（P<0.05）.2.The relative expression of p-Akt and p-P70S6K protein in K562 cells treated with 40mg.L-1DADS for 12,24h was significantly lower than that in control group（P<0.05）.The expression of LC3 protein was significantly higher than that of the control group（P<0.05）,and the expression of Akt,P70S6K were not significantly different（P>0.05）.3.In the DADS group,pathway inhibitor group and the combination group the relative expression levels of p-Akt,p-P70S6K protein were both lower than the control group（P<0.05）;The expression of total Akt,P70S6K protein were not significantly different（P>0.05）.In the DADS group,pathway inhibitor group and the combination group the relative expression levels of LC3protein was significantly higher than that of the control group（P<0.05）and the combination group was the highest group（P<0.05）.Conclusion:DADS induces the autophagy of K562 cells,and its mechanism is related to DADS inhibits the activation of key proteins Akt,P70S6K in the PI3K/Akt signaling pathway. |
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