Effects Of Mipu1 On Autophagy Induced By H₂O₂ In Endothelial Cells

[Object]The aim of this study was to investigate the expression of myocardial ischemic preconditioning up-regulated protein 1（Mipu1）in human umbilical vein endothelial cells（HUVECs）during oxidative stress and to study the effect of Mipu1 on autophagy during oxidative stress.[Method]1.Western blot was used to detect the expression of Mipu1 protein in HUVECs at different concentrations and treatment time with H₂O₂.2.To construct the HUVECs model with Mipu1 overexpression or interference.Then to observe the effects of Mipu1 on HUVECs autophagy in the process of oxidative stress.3.The effects of Mipu1 overexpression and interference on autophagy of HUVECs treated with H₂O₂ were observed by immunofluorescence Assay.4.The effect of Mpiu1 and GFP-LC3 plasmids were co-transfected on autophagy in HUVECs which was detected by fluorescent labeling.[Result]1.The expression level of Mipu1 in H₂O₂ treatment 6,12,24 h group was higher than the control group,（P<0.05）;Mipu1 expression level in 0.25mmol/L H₂O₂,0.5mmol/L H₂O₂ and 1mmol/L H₂O₂ treated group was higher than control group.（P<0.05）;2.The expression of LC3 in HUVECs was increased after transfection with Mipu1 expression plasmid.On the contrary,the expression of LC3 in HUVECs was decreased after transfection with Mipu1 interference plasmid.3.Compared with H₂O₂group,the levels of LC3 and Beclin1 were significantly higher and p62 were significantly lower in the H₂O₂+Mipu1 overexpression group（P<0.05）.However,Compared with H₂O₂group,the levels of LC3 and Beclin1 decreased and p62 increased in the H₂O₂+Mipu1interference group（P<0.05）.4.The expression of LC3 was increased in H₂O₂+Mpiu1+GFP-LC3 group than H₂O₂ treatment group.[Conclusion]H₂O₂ treatment upregulates Mipu1 expression and enhances the activity of autophagy of HUVECs.