| Objective: Human gliomas are one of the most common and most malignant tumors in the long term threat to human health.Treatment of most patients with glioma mainly based on clinical pathology stage of surgery,radiotherapy and chemotherapy and other comprehensive treatment,but the overall effect of treatment is not very satisfactory.Therefore,look for the intrinsic mechanism of glioma progression,finding new effective molecular targets for prevention,diagnosis and therapy in glioma is particularly important.However,the exact etiology and pathogenesis of glioma is still not very clear.EHD2 is encodes a member of the EH domain-containing protein family.This interaction appears to connect clathrin-dependent endocytosis to actin,suggesting that this gene product participates in the endocytic pathway.It extensively regulates multiple receptors in human organs and tissues to further develop their physiological significance.EHD2 is a tumor suppressor gene expressed in some solid tumors,including hepatocellular carcinoma,breast cancer and esophageal squamous cell carcinoma.The study found that EHD2 is a tumor suppressor gene in gliomas,but there is no mechanism for further study.Therefore,in this study,We investigated the relationship between EHD2 expression and tumor migration and invasion,wealso discussedits relationship with glioma progression,and to further explore the possible mechanism.Methods: Through the treatment and detection of 96 human glioma specimens,the expression of EHD2 was significantly lower than that in normal brain tissues by Western Blot analysis in gliomas.The relationship between clinical-related case factors,survival rates and EHD2 expression was also analyzed.And the effect of EHD2 on the migration and invasion of glioma was studied by wound scratch assay andtrans-well assay.Results: The down-regulation of EHD2 was significantly associated with WHO grade(P <0.05),and these patients with low expression of EHD2 showed a shorter survival time(P <0.01).We have demonstrated that EHD2 has a related role in the migration and invasion of gliomas bywound scratch assay andtrans-well assay.Artificial silence EHD2 genecan effectively promote cell migration and invasion by construction of interfering RNA(siRNA)with lowly expressed E-cadherin.Conclusions: Lower expression of EHD2 has vital significance to the progress and prognosis in glioma.EHD2 may inhibit tumorcell proliferation and progression.Therefore,our results suggest that EHD2 could bea favorable independent prognostic indicator of gliomas.Meanwhile,EHD2 not only become a new diagnostic and prognostic markers,but also a variety of malignant tumor treatment of new molecular targets. |
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